The effect of green tea on ultraviolet B-induced sunburn cell production in the skin of hairless mouse

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(1)³¤¥Èó. * .* a. (2005) 45 1 Korean J Vet Res(2005) 45(1) : 1~6. ¶F#`ÒIBJSMFTTîÖÊöB¢%zç^

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(3). *ÎL >~ çL »Ö ]ö¶K\² OÒFÏ\¦ (²Òß: 2005j 2ú 24¢) 1. 2. The effect of green tea on ultraviolet B-induced sunburn cell production in the skin of hairless mouse Sung-ho Kim*, Se-ra Kim, Hae-june Lee, Jin-hee Lee, Yu-jin Kim, Jong-choon Kim, Jong-sik Jang1, Sung-kee Jo2 College of Veterinary Medicine, Chonnam National University, Gwangju 500-757, Korea 1 Department of Animal Science, Sangju National University, Sangju 742-711, Korea 2 Food Irradiation Team, Korea Atomic Energy Research Institute, Daejeon 305-353, Korea (Accepted= February 24, 2005) Abstract : In this study we assessed the influences of ultraviolet (UV) light B radiation on epidermal cells by apoptotic sunburn cell (SBC) and the effect of green tea treatment on the inhibition of SBC formation in SKH1-hr mouse. The extent of changes following 200 mJ/cm2 (0.5 mW/sec) was studied at 0, 3, 6, 12, 18, 24, 30 or 36 hours after exposure. SBCs were recognized by 3 hours after irradiation. There was tendency to increase from 3 hours to 24 hours and decrease from then to 36 hours after irradiation. The mice that received 0, 25, 50, 100, 200, 400 or 800 mJ/cm2 of UVB were examined 24 hours after irradiation. The SBCs were induced as the radiation dose increases from 0 to 200 mJ/cm2. A further increase of radiation dose has little further effect. The frequency of UVB (200 mJ/cm2)-induced SBC formation was reduced by intraperitoneal injection of green tea extract (p<0.01). Key words : ultraviolet B, sunburn cell, SKH1-hr mouse, green tea. * V. ¢>'b UV~ 'f ² UVA, UVB, Ò UVC~ 3¦ªb ¾~Ú æº '' «W >' ßWj &æ ®b 2Ë j> 7ö.æ~ · f Ã&>, º Wª¶*~ Öj 2Z ;ê. B Úö ôf æz¢ &^N . UVC(200-280 nm) º J[ö ~ &¦ª W>>, UVA(320-400 nm) 5 UVB(280-320 nm)ò æRö ê> UV7 UVB º 1-10%¢ Næ . b¦ö & 2Ëê UV~ 'Ë j ÚÚ, UVAº êb~ Fv[, >[ræ 'Ëj. *f ¶F(UV)ö Â>º ~ã ³öB Új& ®b UVº >¦ö s>>w, ï²>w, Ò > ¦z, >¦z ~ ·R >¦>wj ¢bÎ . " ï>«~ Ë" .&ÿ~ Ã& R UV Â~ V²Ã&f z®Ú ~ãJ"ö ~R J[ 2Z 5 ö 8 UV~ .&ï Ã& UVö ~ b¦ æz& Ã&> ®º º^ [22]. \º 8F¦ ö¶K\**Òë~ æöb >¯>îr.. *Corresponding author: Sung-ho Kim College of Veterinary Medicine, Chonnam National University, Gwangju 500-757, Korea [Tel: +82-62-530-2837, Fax: +82-62-530-2841, E-mail: [email protected]]. 1.

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(14) * ÛBö N~ Î. 3. Table 2. Dose-response experiments for sunburn cell (SBC) production 24 hours after UV irradiation UVB dose (mJ/cm2). Number of SBC (mean±S.D.) per cm length of epidermis. 0 25 50 100 200 400 800. 0.33±0.28 28.91±1.81 46.85±13.25 56.16±16.63 81.61±8.68 74.52±9.36 76.89±2.00. Table 3. Effect of intraperitoneal injection or topical application of green tea on UVB-induced increases in apoptotic sunburn cells. Fig. 1. Sunburn cells in SKH1-hr mouse skin after UV exposure. Morphology as seen under the light microscope of (A) untreated skin and (B) UVB irradiated skin with sunburn cells (arrow). H-E stain, X100.. & &V>î 200 mJ/cm j Ò ê 3*¦V ³» B " ; ÖW^W~ ^

(15) îj ßûb ~º SBC (Fig. 1)& &V>V ·~ 6*ö /³® Ã&~&. . 12-24*ö jÝ >&~ ¸f >~¢ Fæ~ & 30*ö £ 50% 6²~ 36*öº /Ï 6² ¢ ¾æî (Table 1). UV Ò ê 24*ö G; UV Ò Ïïê æzº 25 mJ/cm öB¦V SBC& &V> 2. 2. Table 1. Sunburn cell (SBC) production according to the time course after UV irradiation (200 mJ/cm2) Hours after irradiation. Number of SBC (mean ±S.D.) per cm length of epidermis. 0 3 6 12 18 24 30 36. 0.31± 0.49 5.66± 2.67 51.91± 2.47 62.26±24.01 57.87±15.72 61.64±15.25 31.45±15.25 5.91± 3.13. Experimental group. Number of sunburn cells per cm length of epidermis (mean ±S.D.). Normal control Radiation controla Green tea+radiation+green teaa. 0.41± 0.38 74.57±10.74 47.89± 4.06*. Normal control Radiation controlb Green tea+radiation+green teab. 0.31± 0.42 60.77±13.49 50.08± 9.86. The SKH1-hr mice (n=6) were treated with UVB (200 mJ/ cm2) and were sacrificed 24 hours later. a Green tea (50 mg/kg of body weight) or vehicle was given i.p. at 36 and 12 hours before irradiation, and 30 minutes after irradiation. b Green tea cream (0.2%) or cream base (vehicle) was topically treated at 24 hours and 15 minutes before irradiation, and immediately after irradiation. * p<0.01 as compared with radiation control group.. Ú 200 mJ/cm öB &Ë ¸f >~¢ & 25-200 mJ/ cm ~ Ïïræº Ïïö jf~ Ã&~&b¾ 200 mJ/cm ç~ ÏïöB SBCº z ç Ã&~æ p~ b, 400 5 800 mJ/cm ~ ÏïöBê Rb^

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(37) ~ ãÖ F~W ®º Î"º ìî. . N 4~ Î"º Væ >' 5 þ& Ú [30]öBf ? N~ ÖzWªö ~ Î " ºG>¾ ö & º& & ºB .. Ö V. SHK1-hr îÖÊöB UVB Ò ê * ã" 5 ÒÏïê SBC~ ;W æzf N4~ Î"¢ &V ~& . UVB 200 mJ/cm (0.5 mW/sec)j Ò~ 0, 3, 6, 12, 18, 24, 30 5 36* êö æz¢ &V : Ò ê 3*¦V SBC& &V>î 24*ræ Ã&>. & 30*¦V 6²~& . UVB¢ 0, 25, 50, 100, 200, 400 5 800 mJ Ò~ 24*ö æz¢ &V : 200 mJ ræº SBC~ B Ã&>îb¾ ç~ ÒÏïöB æzº ìî . N ;Ú "ÒöB UVBö ~ SBC ;Wf ï~¢ V&b 35.8% 6 ²>î (p<0.01). 2. ^^ò. 1.. B^¢, , B9^. 6îF Ò îÖÊöB N 5 ª³~ OÒF Ë ã6 Î. >~². 5. Bæ^2003, ¢ 43,633-639. BZã, ê\, J8+, ;Æ, J ò, `9, 8, B9, ^. 6îFj Ò îÖÊöB N Ë84~ Î. ]ªç>~²æ 2003, 20, 159-165. f f\ \, «², Iê, ;ã, BÏ, .\, ; 3. > Ï×. NºÂ> Wª catechin ¶Fö , ~ ¶ç* b¦ö ~º 'Ë. ]®*n* 2001, 16, 117-124. `W²æ %, JÒ JÒ¢ ¢, F F . ¶F Òö ~ v b 4. ¦öB~ ¢%zç^~ ;ï' æz. b¦ ²æ 1986, 24, 8-15. 2.. 5. Aberer W, Schuler G, Stingl G, Honigsmann H, Wolff K. Ultraviolet light depletes surface markers of Langerhans cells. J Invest Dermatol 1981, 76, 202-210. 6. Bissett DL, Hannon DP, Orr TV. An animal model of solar-aged skin: histological, physical, and visible changes in UV-irradiated hairless mouse skin. Photochem Photobiol 1987, 46, 367-378. 7. Clydesdale GJ, Dandie GW, Muller HK. Ultraviolet light induced injury: immunological and inflammatory effects. Immunol Cell Biol 2001, 79, 547-568. 8. Cooper KD, Duraiswamy N, Hammerberg C, Allen E, Kimbrough-Green C, Dillon W, Thomas D. Neutrophils, differentiated macrophages, and monocyte/ macrophage antigen presenting cells infiltrate murine epidermis after UV injury. J Invest Dermatol 1993, 101, 155-163. 9. Cotelle N, Bernier JL, Henichart JP, Catteau JP, Gaydou E, Wallet JC. Scavenger and antioxidant properties of ten synthetic flavones. Free Radic Biol Med 1992, 13, 211-219. 10. Daniels F, Brophy D, Lobitz WC. Histochemical responses of human skin following ultraviolet irradiation. J Invest Dermatol 1961, 37, 351-357. 11. Dreosti IE. Bioactive ingredients: antioxidants and polyphenols in tea. Nutr Rev 1996, 54, S51-58. 12. Dufresne CJ, Farnworth ER. A review of latest research findings on the health promotion properties of tea. J Nutr Biochem 2001, 12, 404-421. 13. Fujiki H, Suganuma M, Imai K, Nakachi K. Green tea: cancer preventive beverage and/or drug. Cancer Lett 2002, 188, 9-13. 14. Gilchrest BA, Soter NA, Stoff JS, Mihm MCJr. The human sunburn reaction: histologic and biochemical studies. J Am Acad Dermatol 1981, 5, 411-422. 15. Godar DE. Preprogrammed and programmed cell death mechanisms of apoptosis: UV-induced immediate and delayed apoptosis. Photochem Photobiol 1996, 63,.

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