The Effect of Achyranthis Radix Herbal-acupuncture on Hyperlipidemia in Rats

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경락경혈학회지 Vol.27, No.3, pp.25 46, 2010

∼ Korean Journal of Acupuncture

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Joon-Soo Choi , Yun-Kyung Yim Byung-Ryul Lee , Gi-Young Yang , Jae-Kue Kim

Dept. of Meridian & Acupoints, College of Oriental medicine, Daejeon University Div. of Clinical Medicine, School of Korean Medicine, Pusan National University

Abstract

Objective & Methods : The purpose of this study is to observe the effects of herbal- acupuncture with Achyranthis Radix(AR-HA) at GB34(Yangleungchean) on hyperlipidemia in rats. The author performed several experimental items to analyze the levels of various components and enzymes in serum and liver, as well as the histological changes of liver and aorta.

Results :

1. Achyranthis Radix herbal acupuncture solution increased DPPH radical scavenging activity and HMG-CoA reductase inhibition rate in rat liver cells.

2. AR-HA at GB34 decreased the level of serum total cholesterol, and increased the ratios of HDL-cholesterol to total cholesterol, phospholipid to total cholesterol in hyperlipidemic rat.

3. AR-HA at GB34 decreased the hepatic HMG-CoA reductase activity in hyperlipidemic rat.

4. AR-HA at GB34 increased the hepatic GSH level in hyperlipidemic rat.

Conclusion : From the above results, it is suggested that AR-HA at GB34 has a therapeutic effect on hyperlipidemia.

Key words : Hyperlipidemia, Achyranthis Radix, Herbal-acupuncture, GB34(Yangleungchean)

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- 27 - Table 1. Reagents

Table 2. Instruments

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Scheme 1. Manufacturing procedure of AR-HAS

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Table 3. Composition experimental diet

Scheme 2. Hyperlipidemia experimental animal model

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Scheme 3. Preparation of mitochondrial, microsomal

and cytosolic fractions.

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Fig. 1. Cell viability rate with various concentrations of AR-HAS

Fig. 2. DPPH radical scavenging activity of various

concentrations of AR-HAS

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Fig. 3. Hepatic HMG-CoA reductase inhibition rate of various concentrations of AR-HAS

Fig. 4. Effect of AR-HA at GB34 on body weight in hyperlipidemic rats

Fig. 5. Effect of AR-HA at GB34 on liver weight in hyperlipidemic rats

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- 35 - Fig. 6. Effect of AR-HA at GB34 on the liver index in hyperlipidemic rats

Table 4. Effect of AR-HA at GB34 on various components in serum of rats with hyperlipidemia

Fig. 7. Effect of AR-HA at GB34 on serum total cholesterol level in rats with hyperlipidemia.

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Fig. 8. Effect of AR-HA at GB34 on serum HDL/

total cholesterol level in rats with hyperlipidemia.

Fig. 9. Effect of AR-HA at GB34 on atherogenic index in rats with hyperlipidemia.

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- 37 - Fig. 10. Effect of AR-HA at GB34 on serum LDL- cholesterol level in rats with hyperlipidemia.

Fig. 11. Effect of AR-HA at GB34 on serum phospholipid /total cholesterol in rats with hyperlipidemia.

Table 5. Effect of AR-HA at GB34 on various

enzymes in serum of rats with hyperlipidemia.

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Fig. 12. Effect of AR-HA at GB34 on serum ALP level in hyperlipidemic rats.

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Fig. 14. Effect of AR-HA at GB34 on serum LDH

level in hyperlipidemic rats.

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- 39 - Table 6. Effect of AR-HA at GB34 on various hepatic enzymes in rats with hyperlipidemia

Fig. 15. Effect of AR-HA at GB34 on heaptic HMG-CoA reductase activity in hyperlipidemic rats.

Fig. 16. Effect of AR-HA at GB34 on hepatic glutathione level in hyperlipidemic rats.

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Fig. 17. Effect of AR-HA at GB34 on hepatic catalase level in hyperlipidemic rats.

Fig. 18. Histological analysis of rat liver with

hyperlipidemia(SBB stain) ×200.

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Fig. 19. Histological analysis of rat aorta with

hyperlipidemia(Oil red O stain) ×200.

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