Systemic Aspergillosis associated with bovine viral diarrhea virus infection in Korean native calves
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(2) Ê6""TQFSHJMMVTGVNJHBUVT~ 6" ê'z ÁBÒîÁBÏÁ;B×Á^ÚãÁL ã>~"¦ö B"&v >~" 2 BÞ&v >~"& 3 &v >~"& 4 æ¾ "ã&v (²Òß: 2004j 12ú 9¢) 1. Systemic Aspergillosis associated with bovine viral diarrhea virus infection in Korean native calves Young Hwa Jean*, Jae Hoon Kim1, Dae Yong Kim2, Soon Wuk Jeong3, Oun Kyong Moon, Doo Youn Cho4 National Veterinary Research & Quarantine Service, Anyang 430-016, Korea Department of Veterinary Medicine, Jeju National University, Jeju 690-756, Korea 2 College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea 3 College of Veterinary Medicine, Konkuk University, Seoul 143-701, Korea 4 Department of Veterinary Pathology, Louisiana State University, USA (Accepted=/ December 9, 2004). 1. Abstract : Systemic aspergillosis were outbroken of 17 Korean native calves at livestock farm in Kyunggi province. Two 3 months old calves were died after having diarrhea, coughing, dyspnea, nasal discharge, and abdominal breathing. These calves were diagnosed of aspergillosis as multifocal to coalescing pyogranulomatous inflammation with intralesional aspergillus infiltration at liver, kidney, spleen, heart, lymph nodes, lungs, intestine and cerebrum. Aspergillus fumigatus was cultured from the affected tissues. And the small intestine was positive against bovine viral diarrhea (BVD) virus on FA test. We considered that the secondary fungal infections killed the calves after BVD virus infection primarily. Key words : Aspergillosis, BVD virus, pyogranulomatous inflammation. *. . [3, 10]. Aspergillus fumigatusº ; ÿb º £~ ¾ &~B ÿb 6º ËV* B ~ò¢ Af ÿ böB V²6">Ú ãÖ öÒö ² ~Vê [2]. &¦ª~ .öB ^Vf ã¢ Û 6" >Ú öZ" *Ë&ö ÷æj ¢bÊ, .~¢ Û * > Ë, *Ë&, *, jË" 7ºãê Ë Vöê ÷æj ¢bÎ [4]. Aspergillus fumigatusö ~. Aspergillosisº Aspergillus sppö ~ GW î÷ b &»f b Ò²öê B> Aspergillus fumigatus& &Ë 7º ÷öÚ rJ^ ®, Úö Bº ÷jÒ~ aspergillosisf Ö ÇjæöB Aspergillus fumigatusö ~ öZ.& B : ®æò ÷' B .¾ BVD:Êf~ 6" .º ìî. *Corresponding author: Young Hwa Jean National Veterinary Research & Quarantine Service, Anyang 430-016, Korea [Tel: +82-31-467-1886, Fax: +82-31-467-1800, E-mail: [email protected]]. 93.
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(9) ÖÇjæ~ BVD:Ê 6"" Aspergillus fumigatus~ 6". 95. Fig. 2. Pyogranulomatous inflammation in the renal medulla (A, HE stain, X100), and necrotic center containing thin-walled septated branching fungal hyphae surrounded by necrotic cellular debris, neutrophils, macrophages and occasional multinucleated giants cells (B, PAS stain, X400).. Fig. 3. Pyogranulomatous inflammation in the cerebrum (HE stain, X200), and fungal hyphae (insert, PAS stain, X400).. Fig. 4. BVD viral antigen in the epithelial cells of the small intestine stained by the direct fluorescent antibody test (X100).. . *, æË, Ë f 6ï òWb B'> Ú ® &² V~ Wï ZÒ²& ÖÒ~, ZÒ² "*º &Ú ¯² B'>Ú ® (Fig. 1). ÷Òç' ²Òb ËVöB ÷æ "*ö ^
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(14) ê'zÁfÒîÁf&ÏÁ;B×Á^ÚãÁv. 96. Ò &V>î PAS "ïÖ" 7¦öB G Ò& {>î (Fig. 3). ' ËV~ ÷æ¦öB ªÒ Gº Aspergillus fumigatus {>î ;7Ú ¦ÒÖ" BVD : Êöò ·W>wj &(Fig. 4) PI3, IBR 5 BRS ~ :Êöº rWî .. . V. Aspergillus fumigatusº &»" Ò² Îvö 7º î ÷~ ÷öÚB ²º " ^Vf *Ë&j Û 6 "> .~¢ Û *b *2B [4]. ® B .º « 17v& 6¢ ;ê æ r ~ ÿö B÷~ JÒ, V, »¦ê, j 5 ^ ¦ ~ jÝ ªçÃçj & 7 Ãç 2v& öÒ~ ¦Ò Ö" Aspergillus fumigatus & ö, Ë, Ë, *, jË, *Ë, ò *ËVö 6 ">Ú Gj«W "Ãj ¢bÎ ©b {>î . ² öB~ G~ 6" 㺠G ¦ .~ /& 7º ö¶ ®b, ²öB~ Aspergillusº . *Ë&ö 6"B ê .~¢ æ *b *2B. ~&, ³ËöB «çê¦V / õ ~ 7¦& Gö ~² J">Ú ®º © {> Ú Aspergillus fumigatusº J"B õ j Û ã 6 "B ©b º;>î [1, 5, 7, 8]. öÒ 2vöB & VB ÷Ò' ²Ò" ÿ¢ ªçÃçj © b Ú ³Ë~ ÒG» Îv& ?f Gö 6" >î~ ©b º;>, G~ *ËV~ * º ÷Òç²ÒöB .&~ .*" ãï²Òj ¦*ö Ò& &V>Ú Aspergillus fumigatus& ã 6"B r .~¢ æ *ËV *B ©b 6B [5]. Gö ~ *6" .º VË &~B ç¾, B¢ ËV* ÒÏ ãÖ, n. 6º W. 6²Ã ~ çöB " BB ~ º ® B .¾" ãÖº Ö ê .B Aspergillus fumigatus ë6"ö ~~V º 1N' ² ·Ï~&j ©b º;~& [5, 6]. ³Ëö B B¢ ËV* ÒÏ ' ì «çê ÿö B~& º ÒÖ" &~¢ ¢bʺ 1N' :Ê~ 6" ~>Ú öÒ»ö & BVD, PI3, IBR 5 BRS ~ :Ê ¦Ò¢ ~&, ¦ÒÖ " 2v ÎvöB BVD:Ê ö·Wb {>Ú ²º BVD:Êö ~ K &~B ç öB Aspergillus fumigatus& 6">Ú *ËV ¢ê ©b Òò>î ÏÎ êöB ãV ïb~ ÿ" ÒG~ã æzö V ÊÞ.Êê ? ·Ï~. *6" ç¢ ¾æÞ ©b 6B [6, 10]. ß® Gö ~ òöB~ Gj«W ÷æf Ö ê .B ¦Ò 2v 7 1vöB &V>Ú ¢ B .ö ®ÚB 6"¦ 5 ò¢
(15) Î ËVö & ^& ¦ï 5 Ò& ºB . BVD:ʺ ²zV Ãç", ^V Ãç, ®Ë f K &~ö V ÷öÚ~ 2N 6"j Ï ~² ¢bÊ æ³6"" >6"j ¢bʺ ² ÒG³&ö î' *"W î÷b ÚöBº ²zV Ãçj " ¾æÚîæò "¾öº FÖ ® ËÃçê B> ® [7]. BVD:Ê~ ßû f 6">Úê Ãçj ¾æÚæ p Æ6"çöB æ³'b :Ê¢ VÂ~ î÷j *2Ê [9], :Ê ë6" º BVD:Ê& 6">Ú K &~B çöB 2N' ÷öÚö 6">Ú ãÖ ²& öÒ~Vê ~V r^ö z× "¢ ©b ÒòB .. Ö. . ãVæ Ö ÒG³&~ 3Bú_ Çjæ 17v& J Ò, V, ^ ¦, j 5 ^Ãçj 2v & öÒ~ ¦Ò Ö" Gö J"B õ b¦ V ã6"B systemic aspergillosis {>î BVD :Êö 6">Ú &~B çöB Aspergillus fumigatusö 6"B r Gº .~¢ æ ö, Ë, Ë, *, jË, *Ë 5 &ò *ËV * >Ú Gj«W "Ãj ¢bÎ ©b {>î .. ^^ò 1. Chihaya Y, Matsukawa K, Ohshima K, Matsui Y, Ogasa K, Furusawa Y, Okada H. A pathological study of bovine alimentary mycosis. J Comp Pathol 1992, 107, 195-206. 2. High KP, Washburn RG. Invasive aspergillosis in mice immunosuppressed with cyclosporin A, tacrolimus (FK506), or sirolimus (Rapamycin). J Infect Dis 1997, 175, 222-225. 3. Jean YH, Hwang EI, Kim JM, Kwon YB, Chung UI, Youn CK. Disseminated Aspergillus fumigatus infection in Korean calf. Res Rept RDA(V), 1988, 30, 22-27. 4. Jensen HE, Olsen, SN, Aalbaek B. Gastrointestinal aspergillosis and zygomycosis of cattle. Vet Pathol 1994, 31, 28-36..
(16) ÖÇjæ~ BVD:Ê 6"" Aspergillus fumigatus~ 6". 5. Jensen HE, Schonheyder H, Basse A. Acute disseminated aspergillosis in a cow with special reference to penetration and spread. J Comp Pathol 1991, 104, 411-417 6. Jubb KVF, Kennedy PC, Palmer N. Pathology of Domestic Animals, Vol. 2. pp. 156-157, 4th ed. Academic Press, California, 1993. 7. Radostits OM, Gay CC, Blood DC, Hinchcliff KW. Veterinary Medicine. pp. 1085-1105, 1279-1281, 9th ed. Saunders, London, 2000. 8. Rhodes JC, Jensen HE, Nilius AM, Vhitambar CR,. 97. Farmer SG, Washburn RG, Steele PE, Amlung TW. Aspergillus and aspergillosis. J Med Vet Mycol 1992, 30, 51-57. 9. Schoder G, Mostl K, Benetka V, Baumgartner W. Different outcome of intrautrine infection with bovine viral diarrhea(BVD) virus in twin calves. Vet Rec 2004, 154, 52-53. 10. Shahriar FM, Clark EG, Janzen E, West K, Wobeser G. Coinfection with bovine viral diarrhea virus and mycoplasma bovis in feedrot cattle with chronic pneumonia. Can Vet J 2002, 43, 863-868..
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