The Korean Journal of Internal Medicine Vol. 29, No. 5 (Suppl. 1)
WCIM 2014 SEOUL KOREA 33
Slide Session
OS-IFD-07 Infectious Disease
In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus
Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1
Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2
Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro.
Methods: To test for cytotoxicity of RBV, Vero cells were treated with different con- centrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viabili- ty MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50%
tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcrip- tion-PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey’s post hoc test.
Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/
mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concen- trations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure).
Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment.
Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.
OS-IFD-08 Infectious Disease
West Nile Virus (WNV) as a Potential New Threat to HIV/AIDS Patients in Indonesia
NASRONUDIN1, Bimo AKSONO1, Bimo D LUKITO4, Brian E RACHMAN2, NOORDIANSYAH7, Retno INDRAWATI1, Retno P RAHAYU1, M Inge LUSIDA1 Institute of Tropical Disease, Airlangga University, Indonesia1, Medical Faculty Airlangga University - Dr Soetomo Hospital, Indonesia2, Medical Faculty Airlangga University - Dr Soetomo Hospital, Indonesia3, Medical Faculty Airlangga University - Dr Soetomo Hospital, Indonesia4, Faculty of Dentistry, Airlangga University, Indonesia5, Faculty of Dentistry, Airlangga University, Indonesia6, Airlangga University Hospital, Indonesia7
Background: WNV is a mosquito-borne zoonotic arbovirus, belongs to the genus Flavivirus in the family Flaviviridae. Although WNV infection in human has been re- ported for decades in several parts of the world, but it was diagnosed recently in 2014 in Indonesia. Approximately 80% of WNV infections in humans do not develop any symptoms. Elderly or those with immunosuppression, such as HIV/AIDS, are at greater risk for serious illness. Phylogenetic analysis has divided WNV into mainly Lineage 1(L1) and Lineage 2 (L2), which is geographically specifi c.
Aims: To determine and phylogenetic analyse of WNV on HIV/AIDS Patients.
Methods: The subjects were HIV/AIDS patients in Airlangga University Hospital. Ex- amination of blood samples were performed in Institute of Tropical Disease Airlangga University. This study has been approved by Ethical Committee. A total of 30 HIV/
AIDS patients were enroled after obtainined informed consent, and examined for WNV RNA using RT-PCR in the envelope region (408 bps).
Results: The results showed that 8 (25.81%) of 30 HIV/AIDS patients were WNV pos- itive. The phylogenetic of one WNV strain in this study showed that it belongs to the L2. Only recently were those strains of L2 identifi ed outside of Africa.
Conclusions: We found WNV as one of the potential coinfections in HIV/AIDS patients in Indonesia, and therefore it may in other countries too. Phylogenetic analysis of one strain revealed that the virus clustered in the lineage 2.
OS-IFD-09 Infectious Disease
Low Prothrombin Time is Associated with Increased Ho- mocysteine Serum Level in HIV-Infected Patients
Bernardino ROCA1, Raquel MONFERRER2, Jose A. FERRERO2, Manuel ROCA3 Medicine, Hospital General, Spain1, Biochemistry, Hospital General, Spain2, Ophthalmology, Hospital Provincial, Spain3
Objective: To find factors associated with increased homocysteine serum level in HIV-infected patients.
Methods: Cross-sectional study, carried out as a supplementary task to the usual fol- low-up of HIV-infected patients. The possible association of increased homocysteine serum level with blood analyses results was assessed with a multiple linear regression analysis.
Results: A total of 145 patients were included. Creatinine was higher than normal in 7 patients (5 %), prothrombin time was higher than normal in 36 patients (25 %), and a monoclonal gammopathy was detected in 2 patients (1 %). An association was found between high homocysteine serum level and the following variables: high creatinine (P
< 0.001), low folic acid (P < 0.001), and low prothrombin time (P = 0.007).
Conclusion: An associations was found between high homocysteine serum level and low prothrombin time.
