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In vivo bioluminescence imaging for prolonged survival of transplanted human neural stem cells using 3D biocompatible scaffold in corticectomized rat model

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Figure 1. In vitro luciferase reporter activity in F3-effLuc cells. (A) Retroviral construct that contains the effLuc gene and Thy1.1 (CD90.1), linked with an IRES (internal ribosomal entry site)
Figure 2. Validation of stem cell characteristics in F3 cells and F3-effLuc cells. Flow cytometric analysis showed F3 and F3-effLuc cells are positive for the stem cell surface marker, (a) CD44, and the intracellular marker s, (b) Nestin, (c) Ki67, (d) Sox
Figure 3. In vitro proliferative effect in F3-effLuc cells within the PLLA scaffold. (A) Scanning electron microscope (SEM) analysis was conducted to confirm adhesion of F3-effLuc cells to the PLLA scaffold
Figure 4. Schematic representation of the procedure for in vivo optical imaging. (A) The protocol is for in vivo monitoring of F3-effLuc cells implanted in a corticectomized rat model
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