소(reactiveoxygen species,ROS)를 증가시키게 되는데 이를 알아내는 표식
8주간 사육실험 후의 어류질병에 대한 공격실험 결과는 Fig.9에 나타내었
의 최종대사산물인 암모니아의 농도가 어떻게 변화하는지 알아보기 위해 총 5
요 약 문
병원균에 대한 저항성을 높일 수 있을 것으로 사료되며, 사육수 내의 probiotic (Bacillus spp.)첨가는 암모니아제거 측면에서의 수질정화 효과와 어류의 생존율을 높일 수 있을 것으로 판단된다.
WG (%)1 SGR (%)2 Survival(%) FCR3 PER4 Control 103±5.6a 1.42±0.06a 77.3±15.1a 1.59±0.15b 1.10±0.1a Bacillussubtilis 130±9.3b 1.67±0.08b 97.3±2.3b 1.16±0.05a 1.40±0.0b Bacilluspumilus 121±13.8ab 1.58±0.12ab 98.7±2.3b 1.24±0.05ab 1.30±0.1ab Bacilluslicheniformis 108±14.7ab 1.47±0.14ab 86.7±4.6ab 1.35±0.16ab 1.20±0.1ab Table 3. Growth performance of olive flounder fed 4 experimental diets containing 3 different probiotics for 8 weeks.
Mean values of triplicate groups are presented as mean ± SD. Values in the same column having different superscript letters are significantly different (P<0.05).
1Weight gain (%) = 100*(final mean body weight - initial mean body weight)/initial mean body weight
2Specific growth rate (%) = 〔(loge final body weight - loge initial body weight)/days〕x 100
3Feed conversion ratio = dry feed fed / wet weight gain
4Protein efficiency ratio = wet weight gain / total protein given
Figure 4. The changes of ammonium concentration in the rearing water of olive flounder after a satiation feeding in a preliminary test.
Figure 5. Nitro Blue Tetrazolium (NBT) activity of olive flounder berfore and after the challenge test
Figure 6. Lysozyme activity of olive flounder berfore and after the challenge test.
Figure 7. Superoxide dismutase (SOD) activity of olive flounder berfore and after the challenge test.
Figure 8. Myeloperoxidase (MPO) activity of olive flounder berfore and after the challenge test.
Figure 9. cumulative mortality of olive flounder fed four experimental diets 3 different Bacillus spp. after challenge with S. iniae by immersion (109 CFU/ml).
Figure 10. The effect of probiotics on the changes of ammonia concentration in the rearing water for olive flounder (104 CFU/ml).
Figure 11. The effect of probiotics on mortality of juvenile olive flounder in probiotic treated rearing water after 120 hour.
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