30
-4. DNCB 피부염 생쥐의 멜라닌 생합성 관련 유전자의 발현 변화
이 증가되어 DNCB 군에서 모발성장에 장해가 나타날 것으로 생각되고, DNCB+G군에서 발현이 증가되어 모발 성장이 회복된 것으로 생각된다.
32 -Gene
Counts (average)
log2(FC) P value
DNCB DNCB+G
Krt87 19.29 1207.09 5.96 0.001
Krt81 33.04 2643.65 6.32 0.005
Dsg4 1.03 45.23 5.44 0.001
Hoxc13 17.87 135.92 2.92 0.001
Dsg1b 48.38 4.04 -3.58 0.001
Apcdd1 9.82 48.12 2.29 0.001
Alox12b 54.93 1.25 -5.44 0.001
Table 4. Select skin transcripts related to hypotrichosis in genistein treated DNCB-dermatitic mice by RNA-seq analysis
Krt87 : keratin 87, Krt81 : keratin 81, Dsg4 : desmoglein 4, Hoxc13 :
homeobox C13, Dsg1b : desmoglein 1 beta, Apcdd1 : adenomatosis polyposis coli down-regulated 1, Alox12b : arachidonate 12-lipoxygenase, 12R type.
Gene
Counts (average)
log2(FC) P value
DNCB DNCB+G
Krt87 19.29 1207.09 5.96 0.001
Krt81 33.04 2643.65 6.32 0.005
Dsg4 1.03 45.23 5.44 0.001
Hoxc13 17.87 135.92 2.92 0.001
Dsg1b 48.38 4.04 -3.58 0.001
Apcdd1 9.82 48.12 2.29 0.001
Alox12b 54.93 1.25 -5.44 0.001
Table 5. Select skin transcripts related to hypotrichosis in genistein treated DNCB-dermatitic mice by RNA-seq analysis
Krt87 : keratin 87, Krt81 : keratin 81, Dsg4 : desmoglein 4, Hoxc13 :
homeobox C13, Dsg1b : desmoglein 1 beta, Apcdd1 : adenomatosis polyposis coli down-regulated 1, Alox12b : arachidonate 12-lipoxygenase, 12R type.
35
-Ｖ. 결론 및 제언
제니스테인이 DNCB에 인위적으로 유발시킨 접촉성 피부염 증상에서 모발주 기에 어떠한 영향을 미는지 알아보고자 하였다. 육안적인 관찰에서 DNCB+G군 이 Control군에 비해 피부염 증상은 있지만 DNCB군보다 매우 호전됨을 확인 하였고, H & E 염색에서도 DNCB+G군이 DNCB군 보다 정상군인 Control군에 가까운 표피와 모낭 형태를 확인하였다.
1. 육안적인 관찰에서 DNCB+G군이 Control군에 비해 피부염 증상은 있지만 DNCB군보다 매우 호전됨을 확인하였다.
2. 조직학적 분석에서 DNCB+G군이 DNCB군 보다 정상군인 Control군에 가까 운 표피와 모낭 형태를 확인하였다.
3. DNCB군에서 대조군에 비하여 hair cycle 관련 유전자 Krt71, Dsg4, Msx2, Krtap4-16, Ptch2, Hoxc13, Foxq1 및 Foxn1 mRNA 량이 감소되었고, Krt14 mRNA 량이 증가되었다.
3. DNCB+G 군에서 DNCB군에 비하여 hair cycle 관련 유전자 Krt71, Dsg4, Msx2, Krtap4-16, Ptch2, Hoxc13, Foxq1 및 Foxn1 mRNA 량은 증가되었 고, Krt14 mRNA 량은 감소되었다.
결론적으로 Genistein 투여는 DNCB 피부염의 증상 개선에 도움이 되며, 모발 관련 유전자 발현 변화는 피부염으로 인해서 야기되는 모발의 주기나 성장의 장해의 개선에 도움이 될 것으로 생각된다. 어떠한 기전에 의해서 유전자 발 현의 변화가 일어나는지에 대해서는 추후 계속적인 연구가 있어야 할 것으로 생각된다.
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감사의 글
대학원 석사과정에서 논문이 나오기 까지 많은 분들의 도움을 받았습니다.
논문을 쓰면서 참 너무 감사한 분이 생각이 나는데 이 글을 쓰자하니 표현할 수 없는 감사함과 이 순간이 참으로 낯설기만 합니다.
과연 논문을 무사히 잘 쓸수 있을까 제 자신부터 의구심이 들었지만 류은미 지도 교수님의 가르침으로 석사학위 논문을 무사히 쓸 수 있었던 것 같습니 다. 부족한 저에게 아낌없는 조언과 용기를 주시고 방향성을 잃어가지 않도록 정신적으로 도움을 주신 지도교수님께 너무너무 감사할 뿐입니다. 학업과 직 장생활을 병행하는 것이 쉽지 않았지만 교수님께서 항상 바르게 공부할 수 있 게 해주시고 나약해 질 때마다 늘 응원해 주셔서 힘이 되었습니다. 힘들게 논 문을 구성해 가면서 길잡이가 되어주신 지도 교수님의 조언들이 늘 보탬이 되 었고 인내와 용기 그리고 끌고 나가는 힘을 알 수 있었습니다. 지도교수님께 보답할 수 있는 방법은 교수님의 지도 받는 제자로써 앞으로 더욱 발전 있는 연구를 하는 제자가 되는 것임을 알게 되었습니다. 꼼꼼한 손길로 지도 해주 신 류은미 교수님 정말 감사드립니다. 바쁘신 와중에도 따뜻한 격려와 조언을 해주신 이병래 교수님과 이중헌 교수님께도 진심으로 감사의 마음을 전합니 다.