1. F3 cells were first detected in the contra-lateral side at tumor region 40 min PI .
2. HB1.F3 migrated into tumor region approximately 10 % of total implanted cells in 50 minutes after NSCs injection.
3. In vitro, the doubling time of NSCs was approximately 24 hr, and the proliferative activity of F3, F3.lacZ, and U373MG cells was active for 2 days and decreased thereafter.
4. In vivo, injected F3 cells were continuously found from 50 min to 15 days PI in a same transplantation site. The presence of F3 NSCs was observed in various regions of the whole brain at 15 days after transplantation.
5. F3 cells increased in number over time up to 5 days PI and decreased thereafter.
6. The number of transplanted F3 cells was increased approximately 1.7 fold during the first 24 hrs in the absence of tumor cell inoculation in vivo. However, the proliferation of NSCs began to decline after 5 days following injection.
7. F3 cells (lacZ-labeled) migrate to the tumor region along the corpus callosum.
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8. Several NSCs were observed in corpus callosum & hippocampus but not
found in cerebellum and they were still observed in hippocampus and auditory cortex until 10 days after NSCs implantation.
9. We found that the number of the NSCs increased slowly in a tumor region during 5 days after F3 injection, thereafter the F3 number started to increase dramatically by 15 days. The density of lacZ positive cells in a tumor region was increased until 1 day after injection and thereafter reduced by 15 days after F3 injection.
10. The velocity of NSCs was approximately 0.0175 cm/min.
11. F3 were found in both ipsi- and contra-lateral hemispheres in tumor cell-injected brains but only in the ipsi-lateral hemisphere where F3 NSCs were injected in controls in the absence of tumor cells.
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종양 외의 이주지역, 뇌에서의 증식속도 등을 관찰함으로써 악성신경교종 치료를 위한 전임상 유전자치료에 중요한 정보로 활용될 수 있는 가능성을 볼 수 있었다.
핵심어: 뇌종양, 신경교종, 인간신경줄기세포, 이주, 입체적 분석