상단 PDF Cytoprotective effects Baicalein against oxidative stress-induced cell damage

Cytoprotective effects Baicalein against oxidative stress-induced cell damage

Cytoprotective effects Baicalein against oxidative stress-induced cell damage

1 BACK GROUND This dissertation consisted of two sections, one is the effects of baicalein against the senescence of skin keratinocytes induced by oxidative stresses and the other is the effects of baicalein against the DNA damage of lung fibroblasts induced by oxidative stresses. Lung and skin is typical tissue of human should be exposed to oxygen from the natural environments. According to the scientific development of civilization has been progressed, the natural environment has been destroyed. Human body has the various immune systems against the pollutions of environment, and the immune responses more progressed in tissues which contacted with outer environment. The immune responses generates the ROS and RNS, called free radicals, and they has harmful effects to the normal tissue when the redox balancing was broken.
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Photo-protective effect of baicalein against ultraviolet B-induced oxidative stress

Photo-protective effect of baicalein against ultraviolet B-induced oxidative stress

15 3-3. Baicalein protects DNA, lipids, and proteins against UVB-induced oxidative damage We next investigated whether baicalein can inhibit damage to macromolecules in UVB- exposed cells. First, we monitored UVB-induced DNA damage using the comet assay. The length of comet tails in microscopy images and the percentage of cellular fluorescence in tails are shown in Figure 3A. After treatment of cells with UVB radiation, the comet tail length was distinctly elongated, as well as the ratio of damaged DNA outside of nuclei.
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Protective effect of esculetin against oxidative stress induced cell damage via scavenging reactive oxygen species

Protective effect of esculetin against oxidative stress induced cell damage via scavenging reactive oxygen species

through antioxidant activities [22] . Coumarins in plants are present in the free form or glycosides, and are emerging as potent therapeutic drugs for free radical mediated diseases. Protection of the cellular environment from oxidative stress is dependent on the chemical structure of antioxidants, suggesting that the radical-scavenging effects

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Fermented fish oil derived from mackerel protects skin cell damage by UVB-induced oxidative stress

Fermented fish oil derived from mackerel protects skin cell damage by UVB-induced oxidative stress

UVB irradiation mediates apoptosis by oxidative stress-dependent activation of upstream MAPKs in skin cells [24, 25]. Thus, this result determined whether FFO protects against UVB-mediated apoptosis of skin cells. The present study demonstrated that UVB induced the phosphorylation of MAPKs (ERK, JNK, and p38), which led to apoptotic cell death, whereas the FFO- and MAPK inhibitor-treated cells exhibited enhanced cell viability, suggesting that the protective effects of FFO were mediated by the inhibition of MAPK-induced apoptosis. In addition, FFO showed a more protective effect against UVB-induced cell death than DHA, a main active component of FFO, did and the more potent effect of FFO may be due to the generation of potent antioxidants in the fish oil during fermentation. In addition, our in vivo results demonstrate that FFO prevented the induction of a severe hyperkeratotic epidermis by UVB radiation, and decreased apoptosis-related proteins such as Bax, active caspase-9, and activated caspase-3.
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Propofol protects against oxidative-stress-induced COS-7 cell apoptosis by inducing autophagy

Propofol protects against oxidative-stress-induced COS-7 cell apoptosis by inducing autophagy

reported that H 2 O 2 was associated with cellular toxic effects, including the depletion of intracellular glutathione and ATP, an increase in NAD + level, increase in free cytosolic Ca 2+ , and lipid peroxidation. Among the ROS, the hydroxyl radical can directly attack the DNA backbone by generating oxidative damages, such as oxidized bases, abasic sites, single-strand breaks, double- strand breaks, and DNA-protein crosslinks [22,23]. Via these processes, oxidative stress is involved in the development of cardiovascular, neurological, and oph- thalmological diseases and cancer, as well as in aging [1]. Therefore, we need to develop strategies for pre- venting oxidative injury.
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Mechanism of 2-deoxy-D-ribose-induced β-cell damage : The cystine/glutamate antiporters system c- protects β-cell from oxidative stress

Mechanism of 2-deoxy-D-ribose-induced β-cell damage : The cystine/glutamate antiporters system c- protects β-cell from oxidative stress

Fig. 22. Effects of enforced expression of xCT on the dRib-induced decreases in intracellular GSH content. The cells were incubated with RPMI-1640 media containing 10% FBS in 0, 30 and 50 mM dRib for 6 hours. The intracellular GSH concentration was measured using a glutathione assay kit. Data are presented as the mean ± SD. This experiment was performed in quadriplicate, thrice. **p < 0.01, vs. 0 mM dRib group, as determined by one-way analysis of variance and Duncan's post-hoc test. Control; uninfected RINm5F cells, Empty vector;

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Neuroprotective effects of Carpinus tschonoskii leaves extract against glutamate-induced oxidative stress in HT22 hippocampal cells

Neuroprotective effects of Carpinus tschonoskii leaves extract against glutamate-induced oxidative stress in HT22 hippocampal cells

16 Figure 5. Effect of CTE on glutamate-induced pro-apoptotic relative proteins in HT22 cells. Cells were pretreated with twenty μg/ml of CTE for 12 hr, followed by exposing to 4 mM glutamate. After cell treatment, each groups were maintained in the original medium 12 hr. Equal amounts of proteins in each group were subjected to western blot using the indicated antibodies. Equal protein loading was confirmed by actin expression.

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Effects of propofol-induced autophagy against oxidative stress in human osteoblasts

Effects of propofol-induced autophagy against oxidative stress in human osteoblasts

2 Department of Oral Anatomy, School of Dentistry, Pusan National University, 3 Department of Anesthesia and Pain Medicine, School of Medicine, Pusan National University, Gyeongnam, Korea Background: Oxidative stress occurs during the aging process and other conditions such as bone fracture, bone diseases, and osteoporosis, but the role of oxidative stress in bone remodeling is unknown. Propofol exerts antioxidant effects, but the mechanisms of propofol preconditioning on oxidative stress have not been fully explained. Therefore, the aim of this study was to evaluate the protective effects of propofol against H 2 O 2 –induced oxidative stress on a human fetal osteoblast (hFOB) cell line via activation of autophagy.
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Protective effects of <i>Camellia sinensis</i> fruit and fruit peels against oxidative DNA damage

Protective effects of <i>Camellia sinensis</i> fruit and fruit peels against oxidative DNA damage

Further, PC suppressed ROS production, which protects the oxidative stress-induced DNA damage through reducing H2AX, p53, and caspase-3 phosphorylation. These results refer that the protective effects of FC and PC are mediated by inhibition of p53 signaling pathways, probably via the bioactivity of phenolic compounds. Thus, FC and PC can serve as a potential antioxidant in DNA damage-associated diseases.

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Protective effect of phloroglucinol against gamma radiation-induced oxidative stress in hair follicles

Protective effect of phloroglucinol against gamma radiation-induced oxidative stress in hair follicles

Departments of 1 Advanced Convergence Technology & Science, 2 Veterinary Medicine, 3 Marine Life Sciences, 4 Civil and Enviromental Engineering, and 5 Nuclear and Energy Engineering, Jeju National University, Jeju 64243, Korea (Received: January 22, 2016; Revised: March 13, 2016; Accepted: March 18, 2016) Abstract : When exposed to gamma-rays, hair follicular cells immediately go through apoptosis, which hampers their rapid differentiation essential for the regeneration of hair. Phloroglucinol (PG) is a phenolic compound of Ecklonia cava, brown algae abundant in Jeju island, Korea. Containing plentiful polyphenols, PG is known for its instructive effects by inhibiting apoptosis, scavenging oxygen radicals, and protecting cells against oxidative stress. In this study, we demonstrate that PG rescues radiosensitive hair follicular cells from gamma radiation-induced apoptosis and DNA damage. To identify protective capacity of PG on hair follicles, we irradiated with 8.5 Gy (1.5 Gy/min) of gamma- rays to the whole body of C57BL/6 mice at day 6 after depilation with or without PG. In mice exposed to radiation, the expression of proapoptotic molecule p53 was downregulated in the skin of PG treated group. On immunohistochemical observation of the skin, PG inhibited the immunoreactivity of p53 and cleaved caspase-3. PG treatment protected hair follicular cells from cell death due to gamma-radiation. Our results suggest that PG presents radioprotective effects by inhibiting apoptosis of radiosensitive hair follicular cells and can protect hair follicular cells from gamma-ray induced damage.
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Protective effects of 6'-O-galloylpaeoniflorin against ultraviolet B radiation-induced keratinocyte damage

Protective effects of 6'-O-galloylpaeoniflorin against ultraviolet B radiation-induced keratinocyte damage

UVB, which constitutes only about 4–5% of UV radiation, is thought to be the most active constituent of solar radiation. UVB can reach the earth and penetrate the skin, causing a variety of adverse effects (Afaq 2011). In particular, UV-related skin carcinoma is of great concern, as its rates have been steadily increasing over recent years, and this trend is expected to continue in the future (F'guyer et al. 2003). Keratinocytes are the predominant cell population in the basal layer of the skin, and these cells are the primary targets of UVB (Grewe et al. 1995). The more hazard occurring in keratinocytes or skin tissues after UVB radiation is due to excessive ROS generation. Recent studies have implicated elevated intracellular H 2 O 2 levels in the response to UVB radiation (Chang et al. 2002). In conjunction with previous work regarding the cytoprotective effects of GPF against H 2 O 2 in HaCaT cells (Yao et al. 2013), the observations described above led us to our initial aim in this study: to investigate the cytoprotective effects of GPF against UVB-induced damage in HaCaT cells. DCF-DA staining, a widely used method for intracellular ROS detection, was used to examine the ability of GPF to scavenge intracellular ROS induced by UVB radiation.
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Anti-cancer effects of Lapathoside A from Fagopyrum esculentum in human pancreatic cancer cells and neuroprotective properties of Ecklonia cava ferment extract against glutamate-induced oxidative stress in HT22 hippocampal cell

Anti-cancer effects of Lapathoside A from Fagopyrum esculentum in human pancreatic cancer cells and neuroprotective properties of Ecklonia cava ferment extract against glutamate-induced oxidative stress in HT22 hippocampal cell

According to previous studies, buckwheat seeds contain not only complex carbohydrates, but also many health-conscious ingredients such as minerals, dietary fiber, flavones, flavonoids, phytosterols, and pagopyrins [11-13]. Although compositions of these compounds vary depending on the species, growth environment, these compounds are known to have antioxidant, anti-inflammatory, and anticancer effects [14-16]. However, little is known about the natural compounds present in buckwheat roots. In the present study, lapathoside A was isolated from buckwheat roots as a bioactive compound.
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Cytoprotective effects of KIOM-79 on streptozotocin induced cell damage by inhibiting ERK and AP-1

Cytoprotective effects of KIOM-79 on streptozotocin induced cell damage by inhibiting ERK and AP-1

DISCUSSION This study demonstrates that KIOM-79 protects pancreatic b-cells from STZ induced cell death. Whereas STZ induced the release of ROS, KIOM-79 attenuated this effect, suggest- ing KIOM-79 to have antioxidant properties. STZ stimulates ROS release which is speculated to result in diabetes melli- tus. 3) Most evidence supporting this possibility was obtained from in vitro and in vivo studies, in which STZ increased free radical production, lipid peroxidation, and DNA damage in b-cells, and radical scavengers such as metallothionein, melatonin, quercetin, superoxide dismutase, and vitamin E reduced the severity of the STZ induced oxidative dam- age. 32—38) In the present study, KIOM-79 was found to de- crease intracellular ROS levels, cellular DNA damage, and the lipid peroxidation that is induced by STZ. The cells ex- posed to STZ exhibited the distinct morphological features of apoptosis, including morphological changes and DNA frag- mentation. Cells pretreated with KIOM-79, however, had a significant reduction in the percentage of apoptotic cells.
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Effects of hot water extracts of roasted radish against renal oxidative stress induced by high-fat diet

Effects of hot water extracts of roasted radish against renal oxidative stress induced by high-fat diet

와 protease inhibitor cocktail을 혼합하여 첨가 후 균질화하였다(PT- MR 3100, Kinematica Inc.). 균질액을 1시간 동안 얼음에 보관한 후 4 o C 에서 18,627×g로 20분간 원심분리하여 상층액을 얻었다. 시료의 단백질 농도를 측정한 후 일정량의 단백질이 함유된 whole cell 추출물에 Laemmli sample buffer와 β-mercaptometha- nol 을 첨가하여 혼합하고 이를 sodium dodecyl sulphate-polyacry- lamide gel 에 loading 한 후 90 V 전압으로 2시간 동안 전기영동 (Power Pac300. Bio-Rad) 하여 단백질을 분리하였다. 분리된 단백 질은 니트로셀룰로스 막(nitrocellulose membrane) (0.45 μm pore size, Whatman) 으로 이동시킨 후 5% 탈지우유(skim milk)에서 1 시간 동안 blocking 하였다. 니트로셀룰로스 막을 1차 항체 용액 에 충분히 잠기게 하여 4 o C 에서 밤새 반응시켰다. 이후 세척하여 1 차 항체를 제거하고 2차 항체와 반응시켰다. 2차 항체와의 반응 은 상온에서 1시간 동안 지속하였다. 반응 후 니트로셀룰로스 막 을 세척하여 부착되지 않은 항체를 제거하고, enhanced chemilu- minescence 용액으로 발색시켜 CAS-400SM (Davinch-K, Seoul, Korea) 으로 촬영하였다. 단백질 발현은 image J software (Image J, National Institutes of Health, Bethesda, MD, USA, http://
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Effects of hot water extracts of roasted radish against renal oxidative stress induced by high-fat diet

Effects of hot water extracts of roasted radish against renal oxidative stress induced by high-fat diet

와 protease inhibitor cocktail을 혼합하여 첨가 후 균질화하였다(PT- MR 3100, Kinematica Inc.). 균질액을 1시간 동안 얼음에 보관한 후 4 o C 에서 18,627×g로 20분간 원심분리하여 상층액을 얻었다. 시료의 단백질 농도를 측정한 후 일정량의 단백질이 함유된 whole cell 추출물에 Laemmli sample buffer와 β-mercaptometha- nol 을 첨가하여 혼합하고 이를 sodium dodecyl sulphate-polyacry- lamide gel 에 loading 한 후 90 V 전압으로 2시간 동안 전기영동 (Power Pac300. Bio-Rad) 하여 단백질을 분리하였다. 분리된 단백 질은 니트로셀룰로스 막(nitrocellulose membrane) (0.45 μm pore size, Whatman) 으로 이동시킨 후 5% 탈지우유(skim milk)에서 1 시간 동안 blocking 하였다. 니트로셀룰로스 막을 1차 항체 용액 에 충분히 잠기게 하여 4 o C 에서 밤새 반응시켰다. 이후 세척하여 1 차 항체를 제거하고 2차 항체와 반응시켰다. 2차 항체와의 반응 은 상온에서 1시간 동안 지속하였다. 반응 후 니트로셀룰로스 막 을 세척하여 부착되지 않은 항체를 제거하고, enhanced chemilu- minescence 용액으로 발색시켜 CAS-400SM (Davinch-K, Seoul, Korea) 으로 촬영하였다. 단백질 발현은 image J software (Image J, National Institutes of Health, Bethesda, MD, USA, http://
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Protective effects of Betula platyphylla var. japonica extracts against the cellular damage induced by reactive oxygen species

Protective effects of Betula platyphylla var. japonica extracts against the cellular damage induced by reactive oxygen species

Results Protective effect of methanol extract on H 2 O 2 -induced oxidative damage In order to study cell damage protective effect of a medicinal plant, B. platyphylla var. japonica total methanol extract, we treated cells with chemical and physical stress which cause cellular damage. First of all, in order to determine appropriate H 2 O 2 concentration, H 2 O 2 dose- dependent cell proliferation was measured. Cell proliferation was gradually decreased as the concentration of H 2 O 2 increases (Fig. 1A). Approximately 50% of cell proliferation was observed at 70 µM of H 2 O 2 . When cells were pre-treated with total extract before treatment of 100 µM of H 2 O 2 , the cell proliferation of H 2 O 2 treated cells was increased as dose-dependent manner of total extract treated (Fig. 1B); 106.9 %, 122.1% and 173.7% of cell proliferation at 4, 20 and 100 µg/ml of total extract, respectively.
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Protective effects of red ginseng treated with gold nanoparticles against H<sub>2</sub>O<sub>2</sub>-induced oxidative stress in neuronal PC-12 cells

Protective effects of red ginseng treated with gold nanoparticles against H<sub>2</sub>O<sub>2</sub>-induced oxidative stress in neuronal PC-12 cells

PC-12 세포에 대한 황금홍삼 추출물의 세포 독성 및 세포 보 호능 평가를 위해서 MTT 법을 이용하였다(25). 무해한 농도 결 정을 위한 세포독성 평가는 96-well plate에 PC-12 세포를 2.0×10 4 cell/well 로 분주하여 4시간 배양하였다. 양성대조군은 비타민 C 를 사용하고, 황금홍삼 추출물을 0.00625, 0.0125, 0.025, 0.05 o Bx 의 농도로 세포에 처리 후, 3시간 더 배양을 하였다. 이후 MTT 시약을 첨가하고 3시간 후 DMSO를 이용하여 포마잔(formazan) 을 용해시켜 마이크로플레이트 판독기(Infinite M200)를 이용하여 570 nm 에서 흡광도를 측정하였다. 세포 무독성은 황금홍삼 추출 물을 처리하지 않은 대조군의 세포생존율 대비 80% 이상의 생 존율을 보인 농도로 설정하였다.
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Protective effect of ethanolic extract of antler-shaped Ganoderma lingzhi against oxidative stress in PC12 neuronal cell line

Protective effect of ethanolic extract of antler-shaped Ganoderma lingzhi against oxidative stress in PC12 neuronal cell line

Hyung Don Kim, Eun Young Lee, Jeong-Yong Park, Kyung Hye Seo, Kang-Hyo Lee, Jehun Choi, Jae-Gu Han, Jae-Han Cho*, and Seung Eun Lee* Department of Herbal Crop Research, NIHHS, RDA, Eumseong, Chungbuk 27709, Korea. ABSTRACT: This study was carried out to identify medicinal mushrooms with protective effects against oxidative stress in PC12 neuronal cell line, followed by evaluation of their antioxidant property. Extracts of medicinal mushrooms, including Ganoderma lucidum extract (GLE), antler-shaped Ganoderma lingzhi extract (AGLE), Hericium erinaceus extract (HEE), and Sanghuangporus baumii extract (SBE), were screened for cytotoxicity using MTT assay. None of the extracts up to 10 µg/ml concentration affected cell viability. These extracts were further checked for their protective effect against oxidative stress-induced reactive oxygen species (ROS) production. Exposure to 50 µM H 2 O 2 induced ROS generation in PC12 cells, which was inhibited only by treatment with AGLE. In addition, inhibition of H 2 O 2 -induced ROS generation by AGLE was found to be in a dose-dependent manner (2.5, 5, and 10 µg/ml). Microscopic examination of DCF fluorescence for detection of ROS showed a similar pattern.
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Butin (7,3′,4′-trihydroxydihydroflavone) reduces oxidative stress-induced cell death via inhibition of the mitochondria-dependent apoptotic pathway

Butin (7,3′,4′-trihydroxydihydroflavone) reduces oxidative stress-induced cell death via inhibition of the mitochondria-dependent apoptotic pathway

39. Kang, K.A.; Wang, Z.H.; Zhang, R.; Piao, M.J.; Kim, K.C.; Kang, S.S.; Kim, Y.W.; Lee, J.; Park, D.; Hyun, J.W. Myricetin protects cells against oxidative stress-induced apoptosis via regulation of PI3K/Akt and MAPK signaling pathways. Int. J. Mol. Sci. 2010, 11, 4348–4360. 40. Huang, K.L.; Chen, C.S.; Hsu, C.W.; Li, M.H.; Chang, H.; Tsai, S.H.; Chu, S.J. Therapeutic effects of baicalin on lipopolysaccharide-induced acute lung injury in rats. Am. J. Chin. Med. 2008, 36, 301 – 311.

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Protective effects of Seoritae Chungkukjang added with green tea powder against 3-morpholinosydnonimine-induced oxidative stress

Protective effects of Seoritae Chungkukjang added with green tea powder against 3-morpholinosydnonimine-induced oxidative stress

Abstract : To increase antioxidative activity of Chungkukjang, the protective effect of Seoritae Chungkukjang (SC) added with green tea powder against oxidative stress was evaluated un[r]

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