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RNA interference(RNAi)

RNA Interference of Chitinase Gene in Spodoptera litura

RNA Interference of Chitinase Gene in Spodoptera litura

... 요 약 RNA interference(RNAi)는 살아있는 세포 내에서 유전자의 표현 형을 억제하는 작용을 하고 Chitinase는 곤충이 탈피를 하는 동안 오래된 큐티클의 분해와 재흡수를 도와주는 효소로 알려져 있다. 이러한 작용기작을 이용 하는 연구를 수행하기 위하여 담배거세미나방의 chitinase와 관련하여 탈피저해 효과를 조사하였다. 담배거세미나방 5 령 ...

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Biochemical Characterization of the Drosophila Wingless Signaling Pathway Based on RNA Interference

Biochemical Characterization of the Drosophila Wingless Signaling Pathway Based on RNA Interference

... of RNA interference (RNAi)-based analyses in Drosophila S2R ⴙ cells by using antibodies that specifically recognize Arm phosphorylated at different serine ...

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Effect of RNA Interference-Mediated Suppression of p75 on the Viability of Rat Notochordal Cells

Effect of RNA Interference-Mediated Suppression of p75 on the Viability of Rat Notochordal Cells

... Conclusions: siRNA-mediated suppression of p75 inhibited apoptosis and increased proliferation of notochordal cells under conditions of serum deprivation, suggesting that RNAi might serve as a novel therapeutic approach ...

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Establishment of test systems for evaluating ingestion RNA interference-induced lethality against Tetranychus urticae, a representative sucking pest

Establishment of test systems for evaluating ingestion RNA interference-induced lethality against Tetranychus urticae, a representative sucking pest

... Recently, RNA interference (RNAi) technology has been emerged as a potent tool for pest control ...hairpin RNA by agroinfiltration, COPA(coatomer subunit alpha) gene has been found to be a crucial ...

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Mating Disruption of Grapholita molesta by RNA Interference of a Fatty Acid Desaturase Expressed in Adult Abdomen

Mating Disruption of Grapholita molesta by RNA Interference of a Fatty Acid Desaturase Expressed in Adult Abdomen

... Desaturase, RNA interference 초 록: 복숭아순나방(Grapholita molesta)은 두 가지 주요 성페로몬 성분(Z-8-dodecenyl acetate and E-8-dodecenyl acetate)을 갖고 ...대한 RNA 간섭 처리는 처녀 암컷으로 하여금 사과원에서 수컷을 유인하는 능력을 크게 ...

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Characterization and Functional Analysis of Obox4 during Oocyte Maturation  by RNA Interference

Characterization and Functional Analysis of Obox4 during Oocyte Maturation by RNA Interference

... mega)에 삽입하고 DH5α competent cell에 형질 전환 시켜 다량의 재조합 된 clone을 얻었다. 그런 후 삽입된 유전자가 antisense 또는 sense로 삽입되었 는지를 PCR을 통해 확인하고 in vitro transcription 을 위해 T7 또는 SP6를 promotor로 반응할 수 있 게 Spe I으로 linearization시키고, MEGAscript RNAi ...

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Molecular Genetic Analysis of Spinocerebellar Ataxia Type 7 and a Further Study on the RNA Interference Analysis

Molecular Genetic Analysis of Spinocerebellar Ataxia Type 7 and a Further Study on the RNA Interference Analysis

... 보인다.물론 감소량에는 실험에 따라 편차가 있어 두 RNAi 가 SCA7의 치료에 도 효과적이라고 단정 짓기는 곤란한 듯 보인다.흥미로운 사실은 여러 번의 실 험결과를 거쳤지만 G보다는 A를 타깃으로 하였을 때 보다 효과적이었다는 점이 다.여기서도 타깃과 다른 RNAi 를 처리한 대조군보다는 pREP4벡터를 그대로 사용한 대조군에서 단백질의 감소가 더 크게 나타났는데 정량에 문제가 있어서 일 ...

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RNA Interference

RNA Interference

... B Drosophila S2 cells transfected with plasmids that direct expression of T7-epitope-tagged versions of Drosha,.. CG4792/Dicer-1 and Homeless or untagged -galactosidase.[r] ...

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RNA Interference

The Blocking of TNF-alpha by RNA Interference and Its Influence on Synovial Fibroblast and Chondrocytes

... This study was to use TNF receptor 1 (TNFRI)- specific siRNA to inhibit the TNF-α mediated signaling in RA fibroblast like synoviocytes (FLS) and chondrocytes.. Methods: TNFRI specifi[r] ...

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Comparison of RNA Interference-mediated Gene Silencing and T-DNA Integration Techniques for Gene Function Analysis in Chinese Cabbage

Comparison of RNA Interference-mediated Gene Silencing and T-DNA Integration Techniques for Gene Function Analysis in Chinese Cabbage

... (C) Phenotypic characterization of the KO and KD lines. CON, wild type; KO, BrSAMS-knockout line; KD, BrSAMS- knockdown line. phenol로 처리한 후 원심분리하였다. 원심분리 후 분리된 수 용성 액체에 600μL의 phenol/chloroform을 첨가하고 다시 2차 원심분리한 후, 8M ...

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SELEX with Affinity Chromatography on RNA-RNA InteractionBongrae Cho

SELEX with Affinity Chromatography on RNA-RNA InteractionBongrae Cho

... INTRODUCTION RNA-RNA interaction is a feature of many biological processes including translation, post-transcriptional regu- lation, RNA interference and the action of ...between RNA ...

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vi RNA - In Situ viral RNA

vi RNA - In Situ viral RNA

... ISH rniαuputic1es signæ on light rniαηsco다c in situ hybridization on serni -thick sections of norrnal ælls 하넘 RI-IDVinfec뼈 liver using bio디nyla때 αube.. Infect어 ælls [r] ...

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Synthetic and Adenovirus Delivered Small Interference RNA Pools Targeting Conserved Regions of Foot-and-Mouth Disease Virus

Synthetic and Adenovirus Delivered Small Interference RNA Pools Targeting Conserved Regions of Foot-and-Mouth Disease Virus

... 2 Department of Veterinary Medicine, Chungnam National University, Daejeon, Korea Foot-and-mouth disease (FMD) is an economically significant animal disease because of the speed of its transmission. Routine vaccination ...

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RNA 바이러스

RNA 바이러스

... • 호흡기도 분비물에서 바이러스 항원의 해석은 인플루엔자 감염의 빠른 진 단방법임.. 파라믹소바이러스과(Paramyxoviridae)[r] ...

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Mammalian RNA Granules

Mammalian RNA Granules

... Korea RNA granules such as Stress Granules (SG) and P-Bodies (PB) are aggregates of translationally stalled messenger ribonucleoprotein (mRNP) complexes induced by a wide range of ...of RNA granules, their ...

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RNA Motifs Required for Maintaining Metabolic Stability of M1 RNA

RNA Motifs Required for Maintaining Metabolic Stability of M1 RNA

... M1 RNA Yool Kim, Shinae Suk, Ji Young Lee, Jee Soo Choi, Heegwon Shin, Byong-Seok Choi, and Younghoon Lee* Department of Chemistry, KAIST, Daejeon 34141, ...

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단일 세포 RNA 시퀀싱(Single-cell RNA sequencing) 기술 동향

단일 세포 RNA 시퀀싱(Single-cell RNA sequencing) 기술 동향

... 단일 세포 RNA 시퀀싱(Single-cell RNA sequencing) 기술 동향 박종은 Page 2 / 12 1. 서론 2000년대 개발된 차세대 RNA 시퀀싱 기술은 수천에서 수만 개의 유전자들을 동시에 측정하게 해 줌으로써 생물학의 시야를 급격히 넓혀주었다. 그 이후로 시퀀싱 기술은 발전에 발전을 거듭하여, 하나의 세포에서 수천 개의 유전자를 ...

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ETI(Expected Transmission Interference) for Interference-aware

ETI(Expected Transmission Interference) for Interference-aware

... Ⅴ. 결 론 Wire Mesh Network의 노드는 이동이 거의 없으며 데 이터 흐름이 많고 QoS를 만족시켜야 하는 트래픽이 많 이 존재하므로 데이터 전송을 위한 성능이 좋은 경로를 선택해야만 한다. 본 논문에서는 ETX와 ETT는 채널이 가지는 간섭문제와 WCETT와 MIC보다 향상된 간섭 측 정으로 성능이 좋은 경로를 선택하기 위해 ETT기반의 ETI(Expected ...

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FROM RNA TO PROTEIN

FROM RNA TO PROTEIN

... Eucaryotic fllJr/ bacterial ribosomes are closely related, despite differences ill the number afld size of their rRNA and proteiN components. The rllNA has the domilla11l role ifl transl[r] ...

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Long Noncoding RNA Promotes Triple-

Long Noncoding RNA Promotes Triple-

... RNA pull-down assay To estimate the interaction of miR-93-5p with HAGLR or SRSF1, RNA pull-down assays were conducted. Before that, miR-93-5p sequences with wild-type or mutant seed regions were synthesized ...

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