flow cytometry
Analysis of T Cells Using Flow Cytometry
... Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06520, USA T cells are an essential cellular component of the immune system. When T cells encounter antigen and receive ...
4
Detection of Escherichia coli Using Flow Cytometry
... Key words : Flow cytometry, Escherichia coli, Thiazole orange, Propidium iodide 1) 1. 서 론 일반적으로 대장균의 수를 결정하는 방법은 평판 계수방법 (colony forming assay)이다. 적당히 식힌 한 천배지에 대장균을 도말하여 37 의 배양기에서 밤새 배양한 후 집락된 대장균을 계수하는 ...
11
Multiparameter Flow Cytometry: Advances in High Resolution Analysis
... polychromatic flow cytometry has earned a critical place not only in the identification and enu- meration of immune cell subsets, but also in the assessment of important effector functions such as cytokine ...
12
Quantitative Determination of Plasmodium Parasitemia by Flow Cytometry and Microscopy
... DISCUSSION The success of parasitemia measurement by FACS using a DNA- binding fluorochrome largely depends on discrimination be- tween parasitized cells and other nucleic acid-containing cells in the sample. Because ...
6
Advances in Automated Urinalysis Systems, Flow Cytometry and Digitized Microscopy
... The effort of examining the particles in samples has long been a big issue in the hematologic field [2]. Wallace Coulter devel- oped a counting method for particles in electrolyte solutions [2]. It made accurate cell ...
2
Semi-Functional Quantitative Flow Cytometry Assay for Lymphocytic Choriomeningitis Virus Titration
... using flow cytometry was previously developed but has technical shortcomings, owing to the less optimized parameters such as cell overgrowth, plate scale, and detection ...reported flow cytometric ...
10
Flow Cytometry for the Diagnosis of Primary Immunodeficiency Diseases: A Single Center Experience
... Particularly, molecular tests are essential for identifying the structural abnormalities of genes, thus enabling accurate diagnosis. 7,8 However, genetic tests are time-consuming, laborious and costly. Moreover, targeted ...
14
A New Reporter Vector System Based on Flow-Cytometry to Detect Promoter Activity
... the flow cytometric ...our flow cytometry assay, employing the dual reporter system, has a higher or at least a similar sensitivity to conventional reporter vector systems such as the CAT assay, in ...
5
Effect of Ketamine on Apoptosis by Energy Deprivation in AstrogliomaCells using Flow Cytometry System
... Apoptosis is a programmed, physiologic mode of cell death that plays an important role in tissue homeostasis. As for the central nervous system, ischemic insults can induce pathophysiologic cascade of apoptosis in ...
8
Bone Marrow Flow Cytometry in Staging of Patients With B-cell Non-Hodgkin Lymphoma
... multi-color flow cytometry and examination of bone marrow aspirate are useful methods for assessing bone marrow in NHL ...multi-color flow cytometric analysis can in- crease the sensitivity of this ...
7
Characterization of Human Dental Pulp Cells from Supernumerary Teeth by Using Flow Cytometry Analysis
... The dental pulp stem cells (DPSCs) of SNT from 15 donors showed characteristic of MSCs by flow cytometry using CD markers. The characteristic of MSCs was maintained until pas- sage 10. However, FACS results ...
6
Evaluation of Sperm Sex-Sorting Method using Flow Cytometry in Hanwoo (Korean Native Cattle)
... of flow cytometry, which developed MoFlo Ⓡ suitable sperm sorting or developed form of analyzer combine with sorter ...sperm flow for hydrodynamic ...linked flow cytometry and sex ratio ...
7
Flow Cytometry-Assisted Basophil Activation Test as a Safe Diagnostic Tool for Aspirin/NSAID Hypersenstivity
... a flow cytometry-assisted basophil activation test (FAST) as a safe diagnostic method for ASA/NSAID-induced hypersensitivity, as its high sensitivity and specificity have been demonstrated for many other ...
6
Correlation Between Bone Marrow Blasts Counts With Flow Cytometry and Morphological Analysis in Myelodysplastic Syndromes
... edge: flow cytometry in myelodysplastic ...of flow cytometry results in the WHO classification of myelodysplastic syndromes-propos- al from the International/European LeukemiaNet Working Group ...
4
Single-color Multitarget Flow Cytometry Using Monoclonal Antibodies Labeled with Different Intensities of the Same Fluorochrome
... The most important contribution of SM-FC is that more anti- gen expression profiles can be obtained using multiple mAbs conjugated with the same fluorochromes at different intensities in a multicolor single-tube assay ...
6
Development of a Novel Flow Cytometry-Based System for White Blood Cell Differential Counts: 10-color LeukoDiff
... 11. Allou K, Vial JP, Bene MC, Lacombe F. The routine leukocyte differential flow cytometry HematoFlow method: a new flagging system for auto- matic validation. Cytometry B Clin Cytom 2015;88:375-84. ...
9
Comparison of Two Fluorescent Stain Methods for Jeju Black Cattle Spermatozoa Viability Assessment by Using Flow Cytometry
... 1. Flow cytometry scatter profile of frozen-thawed spermatozoa(A: 6-CFDA/PI staining group, B: calcein AM/PI staining ...후 flow cytometry를 이용하여 forward scatter(FSC)로 세포의 크기와 side scatter (SSC)로 세포 내 ...
6
A Questionnaire Survey of Quality Control of Flow Cytometry in Korea and Development of New Quality-Control Materials
... The purpose of this study was to investigate the current QC status of flow cytometry in Korea through a questionnaire survey, and develop new QC materials using cultured cell lines for[r] ...
10
CD45 is Essential for Lymphocyte Gating in a T-lymphocyte Subset Assay of Bronchoalveolar Lavage Fluid by Flow Cytometry
... 8. Siftar Z, Paro MM, Sokolic I, Nazor A, Mestric ZF. External quality assessment in clinical cell analysis by flow cytometry. Why is it so important? Coll Antropol 2010;34:207-17. 9. Vieillard V, ...
8
Use of flow cytometry to develop and characterize a set of monoclonal antibodies specific for rabbit leukocyte differentiation molecules
... the flow cytometric approach to identifying and characterizing mAbs to rabbit LDMs has shown the pattern of expression of rabbit orthologues of known hCDLDMs have proven, thus far, to be ...color flow ...
16