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Human Umbilical Cord Blood-derived Cells Generate Insulin-producing Cells

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Table  1.  Primer  for  RT-PCR
Fig.  1.  hUCB  cells  differentiated  into  pancreatic-like  cells.  (A)  The  hUCB  MNCs  were  plated  on  0.006%  rat-tail  collagen  (RTC)  coated  coverslips  contained  within  plastic  six  well  culture  dishes
Fig.  2.  Immunofluorescence  of  hUCB-  derived  cells  with  insulin.  (A,  C)  A  negative  control   uti-lizing  isotype-mated   antibo-dies  is  shown
Fig.  4.  Gene  expression  pattern  by  RT-PCR:  The  expression  of  Pax4,  Pax6,  and  Nkx6.1were  increased  and  Ngn3  was  decreased  in  hUCB  derived-cluster  cultures.

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