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Micro-PCR과 Real-Time PCR을 이용한 B형 간염 바이러스 검출강

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Table  1. Primer sequence sets used to amplify HBV-core region for PCR Sequence of primers Sence (5'  → 3') Antisence (5'  →  3') Size of  fragment  Sence ATT TGG TGT CTT TCG GAG TGT G 113 (bp) Antisence GGA CCT GCC TCG TCG TCT AA
Table  2. Comparison of the PCR results conducted on Micro- Micro-PCR with Real-Time Micro-PCR
Fig. 5. In situ monitoring of Real-Time PCR with a broad range of concentration from 1.3×10 3  to 1.3×10 8 copies/µL of HBV plasmid DNA, from left to right (10 8 , 10 7 , 10 6 , 10 5 , 10 4 , 10 3 , N).
Table  4. Comparison of the clinical sensitivity and specificity conducted on Micro-PCR with Real-Time PCR.

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