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Characterization of an alkaline esterase from an enriched metagenomic library derived from an oil-spill area

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Fig. 1 Detection of esterase-positive clones. C, control
Fig. 2 Multiple alignment of the amino acid sequences (A), putative conserved domains (B), and phylogenetic tree of Est7S (C)
Fig. 5 Effect of metal ions on Est7S
Fig. 7 Analysis of enantioselectivity and glyceryl substrate hydrolysis of Est7S. (A) Enantioselectivity analysis with (R)- or  (S)-methyl-3-hydroxy-2-methylpropionate containing phenol red; (B) Hydrolysis of glyceryl esters and oils

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