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In vitro validation of the tear matrix metalloproteinase 9 in-situ immunoassay

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Figure 1.  Scheme of assay protocol (top panel) and kit components and designations (bottom panel).
Fig. 3D). At concentrations > 500 ng/ml, densitometry yielded a saturated signal (Fig. 3D)
Figure 3.  Concentration-band density relationship, as evaluated by densitometry. (A) Using 10 µl of rhMMP- rhMMP-9, a positive test line was detectable at concentrations ≥ 50 ng/ml
Figure 5.  Additional experiments demonstrating the volume-dependency of the MMP-9 immunoassay
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