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Carboxyl-terminus of the amyloid protein precursor and ERB are required for estrogenic effect in activating mitogen-activated protein kinase

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Academic year: 2021

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Figure 1. Gene construction. A, Three constructs. The APP-C105 and hERB were placed under the control of the NSE gene promoter (pNSE/hAPP-C105 and  pNSE/hERB) (a and b)
Figure 2. Expressions of hERB and APP-C105 in transfected cells. Protein  (50 (xg) were analyzed by Western blot using anti-APP antibodies (Zymed)  (A) and polyclonal rabbit anti-ERB (Santa Cruz) (B)
Figure 3. Effect of estrogen on hERB- and APP-C105-mediated activation of the MAPK-signaling pathway

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