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Sphingosine-1-Phosphate-Induced Migration and Differentiation of Human Mesenchymal Stem Cells to Smooth Muscle Cells

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Fig. 1. Role of Gi in S1P-induced migration of BMSCs. (A) BMSCs were loaded onto the upper chambers of the Boyden apparatus and serum-free media containing the indicated concentrations of S1P were placed in the lower chambers
Fig. 2. S1P induces elevation of [Ca 2+ ] i in BMSCs. Serum-starved BMSCs were loaded with 5 μM Fluo-4-AM for 40 min at 37 o C in the absence or presence of 10 μM of VPC23019 and then treated with serum-free medium containing 0.1 μM S1P in the absence or p
Fig. 3. S1P stimulates migration of BMSCs through an S1P 1 -dependent mechanism. (A) BMSCs were pretreated with serum-free medium containing 10 μM of VPC23019 or 1 μM Ki16425 for 15 min, loaded into the upper chamber, and serum-free media containing vehicl
Fig. 4. Signaling pathways involved in S1P-induced migration of BMSCs. (A) BMSCs were pretreated with vehicles, 10 μM LY294002, 10 μM Y27632, 10 μM U0126, 10 μM SP600125 or 10 μM SB202190 for 15 min, and then exposed to serum-free medium containing 0.1 μM
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