J Plant Biotechnol (2019) 46:205 –216 1
Supplementary Table 1 Primers used in this study
Name
1Sequence
2Usage
MG-1065 (S) GActcgagATGGTGAGCAAGGGCGAGG
35S::dsRNAi_eGFP construction (F arm) MG-1066 (AS) GCgaattcATGCCGTGAGTGATCCCGG
MG-1067 (S) TAggatccATGGTGAGCAAGGGCGAGG
35S::dsRNAi_eGFP construction (R arm) MG-1068 (AS) CGaagcttATGCCGTGAGTGATCCCGG
MG-1019 (S) ATGGTGAGCAAGGGCGAGGAG
RT-PCR of eGFP dsRNA MG-1020 (AS) ATGCCGTGAGTGATCCCG
MG-1823 (S) GAGCGCACCATCTTCTTCAA
Genomic PCR of 35S::dsRNAi_eGFP cassette MG-1824 (AS) AACTCCAGCAGGACCATGTG
MG-2330 (S) CATCCATTGCGACCTGAAAC
RT-PCR of LOC_Os01g05960 MG-2331 (AS) AATTTGTGCAGATCCACCGA
MG-2332 (S) GCAGATCAAGGACACATGCC
RT-PCR of LOC_Os01g27600 MG-2333 (AS) ACGAGCATCGAGTTGAGCAC
MG-2336 (S) CATCCATTGCGACCTGAAAC
RT-PCR of LOC_Os01g45410 MG-2337 (AS) AATTTGTGCAGATCCACCGA
MG-2728 (S) GCAGATCAAGGACACATGCC
RT-PCR of LOC_Os01g58240 MG-2729 (AS) ACGAGCATCGAGTTGAGCAC
MG-2831 (S) TGGTGGTGACAGACGAGACC
RT-PCR of LOC_Os01g58270 MG-2832 (AS) TCTGCGTCCGGTACACTGTT
MG-2839 (S) GGACGAGGACGACCTCAAGA
RT-PCR of LOC_Os01g62945 MG-2840 (AS) CCAGACGGACTTGTACACCG
MG-2829 (S) GTCAGGTCGGAGAAGGATG
RT-PCR of LOC_Os01g73720 MG-2830 (AS) GACTTGTACACCGCGATG
MG-2803 (S) TAGACGAGGGCCACAGTT
RT-PCR of LOC_Os02g26160 MG-2804 (AS) CACCGTCAAATGCATCTCTC
MG-2811 (S) GTTCCAGCTCAGCTCGAC
RT-PCR and qRT-PCR of LOC_Os02g57700 MG-2812 (AS) TAGAACTTGAAGCCGAGGTC
MG-2732 (S) GCTCTCTTCTCGGAGCCC
RT-PCR of LOC_Os03g12570 MG-2733 (AS) GATGAGGAGGGGAACAGG
MG-2837 (S) TCTTGGCTTTAGTACTCCGT
RT-PCR of LOC_Os03g12790 MG-2838 (AS) GTTCGCCTTCCATTCCTTC
MG-2726 (S) ACCTGAACGAAGGGGTGAAG
RT-PCR of LOC_Os03g38610 MG-2727 (AS) CTAGCGATGCTGTTGCCG
MG-2722 (S) AACGGCGACGGCGGT
RT-PCR and qRT-PCR of LOC_Os04g52630 MG-2723 (AS) CTGGTCAGCTTGGTTATTCAGAAA
MG-2833 (S) CGGAGGTGATGAGAGTTCTCG
RT-PCR of LOC_Os04g54930 MG-2834 (AS) TTGGTCTCACTTTCATCGGC
MG-2819 (S) CAAGGCTACTATGGGCCCTC
RT-PCR and qRT-PCR of LOC_Os04g56930 MG-2820 (AS) CCAAAAACTGCCACACCAAC
MG-2835 (S) ATGGCGGAGTCGGCG
RT-PCR and qRT-PCR of LOC_Os05g34800 MG-2836 (AS) GTTGATGAGGTCACCGAAC
MG-2817 (S) TCAGGAAAGGAGCAGAAGAA
RT-PCR of LOC_Os06g05230 MG-2818 (AS) TCTCGGCACAAATGTAACAA
MG-2805 (S) ACTGCGTGGAGTGGTCTC
RT-PCR of LOC_Os06g39530
MG-2806 (AS) ATGGTGGCACGATGTCAG
2 J Plant Biotechnol (2019) 46:205 –216
Supplementary Table 1 Primers used in this study (Continued)
Name
1Sequence
2Usage
MG-2823 (S) CAACGGTGAACTTCATGGTG
RT-PCR and qRT-PCR of LOC_Os06g42610 MG-2824 (AS) ACTCCTGCTTCTCGACGAA
MG-2825 (S) GAGGAGGAGGAGGAGGAG
RT-PCR and qRT-PCR of LOC_Os07g10110 MG-2826 (AS) CATATACACTTCTGCGCATCT
MG-2821 (S) AACGACGAGCTCGCC
RT-PCR and qRT-PCR of LOC_Os09g10760 MG-2822 (AS) GTCCACAAATACAGGCTCTG
MG-2809 (S) CTGCTCGAGCACTTCAAG
RT-PCR of LOC_Os09g34930 MG-2810 (AS) GGTCTTGGCGAAGAGGTC
MG-2730(S) TACATCCTCTGCTCCCTCCC
RT-PCR of LOC_Os09g35600 MG-2731(AS) ACGAGGACGACGTTGATGG
MG-2807 (S) ATGCGGACACGGTCG
RT-PCR of LOC_Os10g23830 MG-2808 (AS) ATGAGTCCGGCATGACAG
MG-2813 (S) ATGACGATGGTGGCTGCG
RT-PCR of LOC_Os10g26750 MG-2814 (AS) CTTTAGGCACAGCATCGATC
MG-2815 (S) TAGGGTTGTGGGAAGAAGTT
RT-PCR of LOC_Os11g35200 MG-2816 (AS) CAAAGGTAACACAGACCCTG
MG-2734 (S) ATCGCCCCCTTCTTCTTCTT
RT-PCR of LOC_Os11g41710 MG-2735 (AS) CATGAGGTGGTGCAGGTGG
MG-2724 (S) CTCTACGAGATGGGCACCAA
RT-PCR of LOC_Os11g47350 MG-2725 (AS) ACGCTCACGTTGAAGCTGAC
MG-2338 (S) TCTGCTGCTCATGTACGCAA
RT-PCR and qRT-PCR of LOC_Os12g01910 MG-2338 (AS) CGCACTGATTGAACAAGCCT
MG-2338 (S) CTCCTCCTCCGCCTCTAC
RT-PCR and qRT-PCR of LOC_Os12g27070 MG-2338 (AS) CTTTCTAGGCCAAAGATCCG
MG-2338 (S) AACCACGTCCCCTTCTATC
RT-PCR of LOC_Os12g38090 MG-2338 (AS) CTTGTAGGTGTTTGTGCGAG
MG-0366 (S) GTAAGCAACTGGGATGACATGGAGAA
RT-PCR and qRT-PCR of OsACTIN MG-0367 (AS) CCTCCAATCCAGACACTGTACTTCCTC
1
S and AS in the parenthesis indicate sense and antisense, respectively.
2
