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Extended Culture of Bone Marrow with Granulocyte Macrophage-Colony Stimulating Factor Generates Immunosuppressive Cells

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Academic year: 2021

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Figure 2. Long-term cultured BM cells retain homogeneous levels of CD11c and MHC II. BM cells cultured with  GM-CSF for 1 wk versus (A) 4 wk or (B) 57 wk are compared for their MHC II and CD11c expression
Figure 3. The expression of surface molecules is evaluated on BM cells in the extended cultures with GM-CSF
Figure 4. Cells from the extended cultures of BM with GM-CSF are suppressive to MLR. (A) BM cells cultured for 1 wk (1W-BMC) were mixed with either control  syngeneic splenocytes or BM cells cultured long-term (LT-BMC) for 16 or 32 wk and co-cultured with
Figure 4. (Continued) Cells from the extended cultures of BM with GM-CSF are suppressive to MLR
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