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Vav3, a GEF for RhoA, Plays a Critical Role under High Glucose Conditions

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Fig. 2. (A) Total RNA was cultured under high glucose culture conditions in the presence or absence of 5-aminoimidazole-4-carboxy- 5-aminoimidazole-4-carboxy-amide-1-d-ribofuranoside (AICAR) treatment, and reverse transcription-polymerase chain reaction (R
Fig. 4. (A) C2C12 cells were transiently transfected with 50 nM  siRNA Vav3 for 2 days. Total cell lysates (25 μg) were analyzed  via Western blotting for anti-Vav3 and anti-β-actin antibodies. (B)  C2C12 cells were allowed to differentiate for 7 days

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