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Protective Effect of Heme Oxygenase-1 on High Glucose-Induced Pancreatic β-Cell Injury

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Fig. 3. Cytotoxicity assay of qualifying cell death in INS-1  cells. 5×10 5  cells/well were seeded into a 96-well plate, and  each well was treated with 10 µL of CCk-8 solution (Cell  counting kit-8) for 3 hours at 37°C containing 5% CO 2
Fig. 5. Effect of cobalt protoporphyrin (CoPP) on cell death  rate in cultured INS-1 cells using acridine orange  (AO)/prop-idium iodide (PI) staining
Fig. 6. Intracellular peroxide level in INS-1 cells using flow cytometry. (A) The amount of reactive oxygen species (ROS) was  measured to observe the effect of cobalt protoporphyrin (CoPP)

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