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Characterization and Functional Analysis of Obox4 during Oocyte Maturation by RNA Interference

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Academic year: 2021

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Table 1. Sequences of oligonucleotide primers used in this study and their annealing temperature (AT) and expected RT-PCR product sizes
Figure 1. Differential expression of  Obox family mRNA according to the ovarian, testicular developmental stages and oocytes
Figure 3. Specific suppression of  Obox4 mRNA expression by Obox4 RNAi. Injection of  Obox4 dsRNA into the  GV oocytes markedly suppressed Obox4 mRNA expression, but not Obox3 or Obox5, of which coding sequences are highly homolog with the Obox4 coding seq
Figure 5. Chromosomal configuration by orcein staining after Obox4 RNAi with 2.0 µg/µl dsRNA followed by in  vitro maturation

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