Metabolomics I
⓸ᄅ㔈⊝ቸ? ㎜㔝ಥኰ 㘈౭■ജℼ✈ ⛔⃡♙✵ቸኔ.
Uploadர Ⴘ ቖ╸≥ቸኔ. ✤ ⟀ ⅑ജ≔ឭ఼ ₍ற㔙ቸኔ.
✤ 㔉▀ ⟌ர ⟌ಥ㔈ಐ❀ 㔸ገ ■ജ⟅Ფர ⊍✈ ✤⯘
ኰ⁜■ജ ⚴㔌 Omics✵ቸኔ.
I. Introduction
✨ழ఼ᄸ㒴᠌⟍㉨ ✤㙴▀ ₍㔉ᴴ┬ቄ ⥰၌ ⇈⊝ჴழ✈ ■ജ ᚠ╤ಐ፴ ၘ⛴ 〬✈ ❕
⠡✈ ᯌ➴⛴ ᴸಬ ᨷ ჴ⁜✤▀ ✤ᢘ╤ၸಐ, ⥰ම፴ ඨ◰⛰ ★⃡㔌 ■ജ㘌ᎉ✤ ✤╤⥰ಐ
✸≥ቸኔ. ✨ழ✈ ⥸ᲁಬ ರ៘Ꮜ ▜វ ■ജ ᷴ㔈▜ ✤⟌ቄ ᎉ, ⊍ ᒡ ⠤✈ ■ജ
ጄ♡ ᯌ➴⊌ご ⚴㔌 ⛰ ᄨ៕ᒔ⛴ ♨✤ಐ ✸ቄ ళ ᗀ㔌 ⁜⊔✵ቸኔ. ✤វ㔌 ⊌ኰ⠠
ᤈ Წ ᓼ ⊍✈ ✤⯘ኰ⁜✈ ■ജ▀ ᯌ➴Ꮜ Omics⇐✤ ⟁ᩙᏈቄ ళ⛰ ඩ㜸 ❀■≔
ឭኔಐ 㔐 ⇈ ✸≥ቸኔ.
⊍✈ ✤⯘ኰ⁜✤ᜬ 㔘⛰ ⊍✈ ₍❕㔈ቄጠ 㓴♄㔌 ♄ℼர ⓴ቼ, ⤹ ✬⯘ኰ⁜
(primary metabolism)▀ 㓴♄㔌 㘄㔙✤ ⓴ቼ ☨ᴰ᠌ᴰム✈ ᯙ╤ ᒡ✈ ᩙ➱⛬᠌ 㓴♄▀ ✈ 㔤 ✬⊌➱ 㗩⛰ ⁱ⊌➱⛬᠌ ⊍▀ ✈㔤 ᒔ╤⥰ቄ 㘄㔙⛴ ✤ᤤಐ ✸≥ቸኔ. ♠ᥜர ✬ ⁱ₍㘌▀ ✤ ⁜♙Ꮘಐ ✸ቄ drug, dye, flavor, fragrance, cosmetic▀ ⣁♄㔌 ⚀ர Ꮘ╤
☄⛬᭰᠌ ✨ழ✈ ₍㘌▀ ᦔ♠ ⣁♄㔌 ♄ℼᒔ✤ᜬಐ 㔐 ⇈ ✸≥ቸኔ. ♠ᥜர ∭఼ ✤㔤㔐
⇈ ✸ቄ ⟌㐸⛬᠌ቄ ⓴≔㓬ᥠ✤၈ ろ⅄ ᒡ⛴ ኰ㐌➱✨ ▸᠌ ᒔ ⇈ர ✸≥ቸኔ. ✤ិ఼ ⣁
♄㔌 ⚀ᒔ⛴ ⊍▀ ╫ ⚴㔌 ᄨ៕ᒔ✤ ඨழ ⛰ ╵ಬ 㔉❀ᒔ▀ ✈㔤 ₍ 㗩⛰ ■ ജᏈ╤ ☄≥ቸኔ. ඨវ၈ ✬ᴰ ኰ⁜ᒔ⛰ ♠⇈㔌 㙘ቕ▀፴ ᴸജ㔈ಐ ၞ⛰ ⇈⛘ಬ ᴴᥜ⟅
⟌✈ ╤។♰⛬᠌ ⁱ╵㘄▀ ✤ᤤ⥰ ᩫ㔈ಐ ■ജ၈ ₍✤ ᲤᤈᏌ ▸ர ⁱኩ㜸 ✤ ✸≥ቸኔ.
㔝┄⟌᠌ ⓼។⟨ ✸ቄ vinblastine✤၈ vincrisitine፴ ▝⊌ ✤វ㔌 ᱄⢬▀ ℽ㔈ᨠ ✤ ౭♠፴ ⁱ╵㘄▀ ⥰ቄ ⁱ㔌 ᯄ◰ ✤ ၞ⛰ ₍⃡⛬᠌ ✨㔤 ✤ᤤ⥰ ᩫ㔈ಐ ✸≥ቸኔ. 80ჴኰ
⥰ 㔤፴ ✤វ㔌 ၞ⛰ ⇈⛘✈ ⟌ 㔤ౠ㔈 ⚴㔤 ☨ᴰ▀ elicitor ⱘர㔤 ⢬ఠ၈ ᯠ⥰✈ ⠠⃡⛴ ᱰ㘄⊌ごቄ ᒡ✈ ☨ᴰ㘈౭✨❀✈ ᱰ㘄 ⢬᠌ 㔈▜☄⛬၈ ✤⟌ቄ ✤វ㔌 ⟁ ඬᯙ᱅▀ Omics✈ ኔ╁㔈ಐ ┎⃐ ⇐ᒔ⛴ ⟁ᩙ㔈▜ Ფኔ ඬ⚀➱✨ ⟌㔤ౠᄨ៕⛴ ♨▜
┬ 㔌ኔಐ ₍ற㔙ቸኔ.
ඨវ ⚴㔤ቄ Omicsᜬ ᨵᨵᏈ╤ 㘼❀Ꮘಐ ✸ቄ ♙╤▀ ኰ㔌 ✤㔤ர ♠⃐➱⛬᠌ 㓴♄㔈 ኔಐ 㔐 ⇈ ✸≥ቸኔ. ✤វ㔌 ⇐▀ ኰ㔌 ✤㔤 ᯄゅ⛬᠌ றற✈ Omics ኰⁱ₍▀ ➱
➸㔈఼ ➱♙㔈ቄ ✁♙៕⛴ ⵅ➱㔈▜┬ 㔈ಐ ඨళ✤ ඨழ bottleneck▀ ᱇╤၈ಐ ✸⥰ ᩫ 㔌 ᨷᨷ ಥ⟅ᒔ⛴ ඬ⚀➱⛬᠌ ௌ⃐㔐 ⇈ ✸ቄ 㔤ౠ⯵⛬᠌ ⟌⊌Ꮠ ⇈ ✸⛴ ళ✵ቸኔ. 㓴❀ቄ
✤វ㔌 ᩙ➱⛬᠌ ⊍▀ ኰ㔌 Omics ⃔ᨵᒌᥜಐ❀ 㔈ᨠ ✤ Ⲹ᠌ ற❀✈ ⊌≔ㄌ▀ ➱
➸㔌 ✁♙⛴ 㔈⊌ ᯄቸኔ.
I-1) Metabolome
Analytical
Kinetic and Flux
‘Omics’
S Definitions
Metabolome
Com ge
Pathway, Network and
Interrelationship of Key Technologies used for Metabolic Pathway Engineering in Post-Genomics Era
Micro, In situ
Metabolome⛰ ኰ⁜Ɽ ✈ᮨ㔈ᨠ ⊍✤ ₍㔈ቄ ᩘᒐ ኰ⁜⥸✈ ⳍ⸝⛬᠌ ✤㔤㔈⊌ᨤ
∜♨ ళഠ♄. ✤វ㔌 ௌᄀ➱ ✤㔤ቄGenomics, Transcriptomics, Proteomics✈ genome, transcriptome, proteomeಬ ᷴഀ㔤 ✤㔤㔈⊌ ᯄቸኔ. Functional Genomicsቄ ற ⛐➴❀
ᯍ㖰⥴ gene sequence ர ඨ ✈ᮨ ⯮ ⚴㔤
❀■≔ឭ఼ ■ജᯙ㕕✤
Functional Genomics᠌
✤ᢘ╤⥰ಐ ✸≥ቸኔ.
⛐➴❀ᒔ✤ ඨ ቕ⛴
㔈఼Ꮘᨤ mRNA, protein (enzyme), metabolite✈
⇌᠌ ᴰ ✤ ⯭ⵌᏈ ಐ ✤ᒔ✤ ➱➸㔌 ቕ
⛴ 㔈▜ organism✤ 㘈 ౭▀ ➱✁㔈ᨠ ⓴ஸ
⇈ ✸፴᠍ 㔘⛰ ⢬⥰✈ ⁜⊔✵ቸኔ. ᓠᜬ ╤ᕔ ⛐➴❀✈ ቕ⛴ ㊼⓵㔈 ⚴㔤ቄ mRNA, Protein, Metabolite▀ ኰ㔌 ᴴ ᮿ ■ജர ᯈᒌ⊌ 㓴♄㔙ቸኔ. Metabolome⛴ 㔌ᓄ
᠌ ⟅✈㔈ᨤ 㝣end products of gene expression㝤✤ᨠ ᎉ⊌▀ 㝣biochemical phenotype of a cell or tissue㝤ᜬಐ 㔐 ⇈ ✸≥ቸኔ.
♠ⷑ✈ ፴
Methods
equences Profiles
Genes Proteins Functions Parallel, Ultra-
sensitive plete Ran
Systems Engineering
✈ ቕ⛴ ඌᨵ㔈ቄ 㔉⛴ ✈ᮨ㔈ᨠ Human Genome Project ✤㙴▀
㐌 Ფ⊌ᨤ Omics
✸
lo ⵅಬ ᴴᯙ᱅ⵅ, ඨᥜಐ kinetics & fluxⵅ✤ ≥ቸኔ.
✤ ⃨ ⵅ✤ ➱➸㔌 ூ⛴ 㘅Ფ 㔤┬ ⚀㔈ቄ ⟅Ფ ╫╤ၬ
⇈ ✸ಐ ඨᝈ┬ ⚀㔈ቄ ╁✈
product ജ㔐 ⇈ ✸⛴ ళ✵
ቸኔ. ₍㘄ಥ✨ᒔ✤ ླྀཨቄ ኰ⁜Ɽ㔉(metabolomics)✈ റ ඩ➱✨ ᩙ➱⛰ systems bio gy
ఠ၈ metabolic engineering✴⛰ ⢬⥰✈ ⁜⊔✵ቸኔ. ඨវ ⚴㔤ቄ ╤ᕔ ₍Ɽ✈ gene expression productᒔ✈ ரቕ㔌㔌 ⛰ profiling✤ 㓴♄㔙ቸኔ. ┎▀፴ ✤ᮨ ╨ඹ⛴ 㔸╸⥰
, mRNA, protei ⛬᠌ቄ Systems biology✈ ᩙ➱✨ “simultaneously monitoring all biological processes operating as an integrated system”⛴ ኜ⃡㔐 ⇈ ╶≥ቸኔ. ኩ■㜸 gene expression
✈ ⴌ⠵✨ metabolome✈ ⟅Ფர 㓴♄㔈⢐. ⥰၌ 6ჴழ✈ Omics✈ ■ജᎉ㕕⛴ ♄┭㔈 ᨤ ⓴ᝈ ඨᥬಬ ≥ቸኔ.
n
(Lloyd W. Sumner et al., Plant Metabolomics: large-scale phytochemistry in the functional genomics era, Phytochemistry, 62, 817-835, 2003)
⚴ ፴㐌 Ფ⊌ᨤ ✤㔤㔈⊌⥰, metabolomics▀ ኰ㔌 ■ജ⃡ಬ✤ ኔᤨ omics▀ ᷴ㔤 㖴➰㔈఼ ➱⛰ ళ⛴ ⓼ ⇈ ✸≥ቸኔ. 2000ჴ፴ ✤㙴ᴰム 㔈ඹ⇈➱⛬᠌ ቈ╤၈ಐቄ ✸⛬၈
▜➴㜸 ჴழ 60▜㍨✈ ᄬ✤ ᯌ㐌Ꮘಐ ✸≥ቸኔ. ✤ቄ ✤ ᴴ┬▀ ኰ㔌 㔉➱ ▝⁜ர ◔ ᝈᏈ╸⛼▀፴ ᴸജ㔈ಐ Ɽ➱✨ ■ജ⦁⣁፴ர ⥍ர㔌⥰ ╬Ꮘ⥰ ⓺┈⛼⛴ ✈ᮨ㔈ቄ ళ✵
ቸኔ. ඨ〬 㔤┬㔐 ✬፴ ಐ ╤។♰✤ ✸⥰, ഝၤ✈ ₍㘄ಥ㔉❀ᒔ✤ ⃐⥴ഝ✈ ₍
㘄ಥ㔉❀ᒔಬ 㔌 ౘᢴᲴ⥱㔌 ᴴ┬ர ⓴ኀ ⇈ ╶≥ቸኔ. ኔᤨ omics✈ ■ജர ✬⟅ ✤ⁱ
✈ ኘ▀ ✤ᤤಐ ✸⛬᭰᠌ ⴌ⠵⛰ metabolome▀ ኰ㔌 ■ജ ▝⊌ ඨ 㗅㍹⃡⛴ ெⵄ╤
┬ 㔐 ళ✵ቸኔ.
