Nuclear Anomalies, Chromosomal Aberrations and Proliferation Rates in Cultured Lymphocytes of Head and Neck Cancer Patients

Lymphocyte Nuclear Anomalies, Chromosomal Aberrations and Proliferation in Head and Neck Cancer Patients

Asian Pac J Cancer Prev, 15 (3), 1119-1123

Introduction

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1School of Bio Sciences and Technology, VIT University, ²Government Vellore Medical College Hospital, Vellore, India *For correspondence: [email protected]

Abstract

Head and neck cancers (HNC) are extremely complex disease types and it is likely that chromosomal instability is involved in the genetic mechanisms of its genesis. However, there is little information regarding the background levels of chromosome instability in these patients. In this pilot study, we examined spontaneous chromosome instability in short-term lymphocyte cultures (72 hours) from 72 study subjects - 36 newly diagnosed HNC squamous cell carcinoma patients and 36 healthy ethnic controls. We estimated chromosome instability (CIN) using chromosomal aberration (CA) analysis and nuclear level anomalies using the Cytokinesis Block Micronucleus Cytome Assay (CBMN Cyt Assay). The proliferation rates in cultures of peripheral blood lymphocytes (PBL) were assessed by calculating the Cytokinesis Block Proliferation Index (CBPI). Our results VKRZHG D VLJQLÀFDQWO\ KLJKHU PHDQ OHYHO RI VSRQWDQHRXV FKURPRVRPH W\SH DEHUUDWLRQV &6$V FKURPDWLG

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visible spontaneous genomic instability and low rate proliferation in the cultured peripheral lymphocytes of solid tumors could be biomarkers to predict malignancy in early stages

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Nuclear Anomalies, Chromosomal Aberrations and Proliferation Rates in Cultured Lymphocytes of Head and Neck Cancer Patients

Alex George

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, Vikas Bhuria

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Materials and Methods

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Clinical and pathological observations

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Analysis of chromosomal aberrations

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Lymphocyte Nuclear Anomalies, Chromosomal Aberrations and Proliferation in Head and Neck Cancer Patients Statistical analysis

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Discussion

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