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FSL-1, a Toll-like Receptor 2/6 Agonist, Induces Expression of Interleukin-1α in the Presence of 27-hydroxycholesterol

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Fig. 1. Expression of TLR6 in THP-1 cells in response to cholesterol  and 27OHChol. (A) THP-1 cells (1×10 6  cells/60 mm culture dish)  were serum starved in 0.1% BSA (endotoxin free) in RPMI 1640  for 24 h and treated with cholesterol (5 μg/ml) or 27OHCho
Fig. 4. Effects of inhibitors of MAPKs on TLR6-mediated expression  of IL-1α. (A) Serum-starved THP-1 cells were treated with 27  OHChol (2.5 μg/ml) for 24 h after pre-incubation for 2 h in the  absence or presence of the indicated inhibitors (5 μM each)

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