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Quality Changes in Minced Ginger Prepared with Frozen Ginger during Storage

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(1)KOREAN J. FOOD SCI. TECHNOL. Vol. 36, No. 6, pp. 943~951 (2004). ©The Korean Society of Food Science and Technology. ïÿ;b‚ *`‚ &8~ Ë 7 ®îæz Bÿ^Áš¾* %v ®". Quality Changes in Minced Ginger Prepared with Frozen Ginger during Storage Dong-Ho Kim and Young-Chun Lee* Department of Food Science and Technology, Chung-Ang University As storing ginger roots under optimum conditions takes high cost, ginger roots are commonly stored in underground tunnels where the maintenance of optimum temperature and humidity is difficult. One of the methods for long term storage of ginger roots is freezing. The objective of this research was to evaluate effect of storage temperatures and packaging methods on the quality of minced ginger prepared with frozen stored ginger. The minced ginger prepared with frozen stored ginger at −20oC was packed in bags, glass bottles and tubes, and then stored at 5 and −20oC for quality evaluation at 4 and 15 week-intervals. The changes of surface color, total free sugars, free amino acids and volatile compounds were less in the combined treatment samples than in control during storage, regardless of the storage temperature. The tube packing was the best for maintaining quality of minced ginger during storage among tested packaging methods. Sensory results showed that the minced ginger with the combined treatment and packed in tubes could be stored at 5 and −20oC for 12 and 45 weeks, respectively, without a significant drop in palatability. Key words: minced ginger, packaging methods, frozen ginger. *. †.   žÿK~ " º²‚ ž‚ Öj Ã& öžš > ® . æ‚ ;~ ç®W Ãê" ¦&&~ Ã&¢ *šB º ^¿, Ú, ïË-ïÿ&Ë 5 š– " ?f î‚Ú *&  &ËO»ö &‚ ’& º’B . “Ú ;~ ²jº ª Z š– ªö" ;¾šÊÞ ~ ¢¦ &B®j Bž~š &¦ªš  ;~ ;‚ Ïb‚ò šÏ> ®Ú ²j ¾& ‚;>Ú ® . $‚ &B® 5  &ËO» BBš š Úææ pj ; ²j~ >º{&ö ‚ê& ¾æ¾ ® .  ’~ Ï'f >{B ;~ ËV&Ëj *~ ïÿ& ËB ;j öò‚ ~, ; &V~ &Ë ö ^B6b‚ ¾æ¾º .æ, &ÊB 5 ‡ªÒ ÛBB¢ Î&‚ ;. &V¢ B–~, ËO»" &ËNê& ; &V~ ®îö ~º 'Ëj ï&~ ïÿ&Ë ;~ ë' šÏ Onj Îï~¶ ~& .. ;(Zingiber officinale Roscoe)f ;"(Zingiberaceae)ö ³ ~º j& $º & öÖ~ j . b~ ~¾‚B ~ "ãj “~Vê ‚ (1). "ãf ßF~ " ËV¢ æ î ®Ú Vö* 3^Vã¦V ^ê'b‚ 6Ò †Ï> ® º V^Wš ±f Ëò~ ~¾š . ÖÒ ¾¢~ ; C  Öïf * £ 4ò Êb‚ ÏÎ BÖ" *§ j" æö B *“ C Öï~ 86.4%¢ ÖÂ~ ®b– “Ú ;~ Ëκ £ 1,500Ûöb‚, Ö ³&ö n;B ²j B ~º 7º‚ ² ·bš (2).  ;&Ë~ ‚'–šf 10-15oC, 80-90% RHž–, ƚöBº  ;j &ˆ H Æ šç& jã~æ á~š ¦NN" BjNš ¸j ; Ë ò~ >/ç ôf ^B6š B‚ (3). šf ?š *Ò “Ú öB ÒÏ> ®º ƚ&ËO»f ÎN'ž &Ë &Ò& Ú J֖ $‚ &ËV*~ .Gš ®&Ë~ "ÖæöB Úê 'š Ëÿ'ž ê³ Â~& > ®æ pj öò jî¢, «. Òò 5 O» ; &8~ *`, Ë 5 ËO». þö Òς ;f 2000j 11ú Öæž ÏÓÎê B֖ ¦CšöB ’«~ ÒÏ~& . ;f −20oC ïÿ &Ëö 1Bú & ê šÿ~ ; &V Òò‚ ÒÏ~& . ; j îê‚ ê ZÎ&–j &–’(control)‚ F;~&b– îê ; &V 100 g 7ïö &‚ jN‚ .æOæB‚º L-cysteine. *Corresponding author: Young-Chun Lee, Department of Food Science and Technology, Chung-Ang University, Naeri 72-1, Daedukmyun, Ansung, Kyungki-do 456-756, Korea Tel: 82-31-676-2451 Fax: 82-31-675-4853 E-mail: [email protected] 943.

(2) ‚“®"²æ B 36 ² B 6 ^ (2004). 944. z¢ rjV *~ DB-5(50 mÜ0.2 mm, 0.33 µm, J&W Scientific, Folsom, USA)

(3) "š ËOB gas chromatography(GC, Hewlett-Packard 5890, Avondale, USA)¢ šÏ~ G;~& . J6Nêº 50oCöB 2ª* Fæ‚ ê ª 5oC‚ 240oCræ çß~&b–, š NêöB 10ª* Fæ~& . ¦ÂVº flame ionization detector¢ ÒÏ~& "«’~ Nêº 250oC, ¦Â V~ Nêº 280oC‚ ~& . GCö ~~ ªÒB ËVWª~ ÿ;f gas chromatography-mass spectrometric detector(GCMSD, Hewlett-Packard 5890, Avondale, USA)¢ šÏ~ G; ~& .

(4) "f DB-5MS(50 mÜ0.2 mm, 0.33 µm, J&W Scientific, Folsom, USA), J6Nêº 50oCöB 2ª* Fæ‚ ê ª  5oC‚ 240oCræ çß8 . GC-MSD‚ áf mass spectrumj Wiley library‚ ¦ï‚ Ö"f ¾^æº ^òç~ mass spectral data(7-9)¢ šÏ~ jv~& .. 0.2%, &ÊB ÛBB‚º NaCl 2%f sodium benzoate 0.1%, ‡ªÒOæ¢ *‚ Î&B‚º xanthan gum 0.1%¢ b Î &~  îz‚ ©j «¾Ò’‚ F;~& . B– B &Vº nylon/polyethylene bag, glass bottle, tube(aluminium foil/linear low density polyethylene)ö Ïê~ ïË&Ë (5oC)òº 4" *Ïb‚ 16" ÿn, ïÿ&Ë(−20oC)òº 15" *Ïb‚ 60" ÿn &Ë ~šB ®îßWj –Ò~& . ‚šïê ª7ïNê(color difference meter, Hunter lab., CQ-1200x, USA)‚ L8(lightness), a8(redness), b8(yellowness)j reflectance modeöB ïê¢ G;~&b– *>'ž ïN ∆E = 2 2 2 ∆a + ∆b + ∆L 8b‚ ¾æÚî, ‚&Wï6~ calibration 8f L = 93.36, a = −0.97, b = 0.43šî . FÒ Žï Gancedo (4)~ O»ö 8¢ FÒ Žïj G;~& . ¯, ''~ ; &Vò 20 gj ~ 80% ethyl alcohol‚ ºÂ ~ 6{³»j Î ê Sep-Pak C18 cartridge(Waters Co., Milford, USA) 5 membrane filter(pore size 0.45 µm)‚ ³'b‚ "~ Sugar-Pak(6.5Ü300 mm, Waters, Milford, USA)

(5) "" R.I. detector(Model 132, Gilson Co., Villiers-le-Bel, France)& Ë OB HPLC(Gilson 305 system, Villiers-le-Bel, France)‚ ªC~ & .. Ë¦Ò ; &Vò~ ïz, š 5 «' ®îö &š ßW Nš¦Ò 5 F^ê ¦Ò¢ ~& . &˦Òö~ FBj *~ b& 36 ¦Ò»b‚ ; &V~ ïz, šö &‚ Nšê ËKš Ö>‚ N6 20«j FB~& . ïz~ &Ë ï&º 96 ï6», šº 76 ï6», *Ú'ž F^êº 9 6 V^¿ê»ö ~~ ¦Ò~& . Ûê¾Ò. þÖ" áÚê ¶ò¢ SAS program(10)j ÒÏ~ ªC ~&b–, ªÖªC‚ Ö" ò*~ Nš& ®º “Ïö &š Bº Duncan’s multiple range test‚ ò*~ F~N¢ ¦;~ & (p<0.05).. FÒ jžÖ FÒ jžÖ ªCj *~ ‚(5)~ O»ö 8¢ ò¢ * ¾Ò ~& . ; &VòöB 20 gj ~ 75% ethyl alcohol χb‚ FÒjžÖj ºÂ‚ ê "~ ‡j 6 {³»Î Ê loading buffer solution(20 mM borate buffer, pH 9.5)b‚ 10 mL‚ ;Ï~ š¢ membrane filter(pore size 0.45 µm)‚ "~& .  ‡" FêÚ £(o-phthaldialdehyde) j 1 : 5‚ &~ 1ª ÿn >wÎ ê LiChrospher 100RP-18 (4Ü250 mm, Merck, Darmstadt, Germany)

(6) "" Fluorometer detector(Model 121, Gilson Co., Villiers-le-Bel, France)& ËOB HPLC(Gilson 305 system, Villiers-le-Bel, France)‚ ªC~& .. Ö 5 V ‚šïê~ æz −20oCö ïÿ&˂ öò;b‚ ; &V¢ B–‚ ê 5, −20oC &Ëö 16, 60" ÿn &Ë  ‚šïêº Table 12ö ¾æÂ :f ? . 5oC &˖öBº &ËV*š ^Úî> ƒ ËO»ö ç&ìš Î ¾Ò’öB a8š Ã&~&b¾ &–’öBº Â2˾Ғ& ž ˾Ғ a8~ æ z& &Ë 8"¦V n;'b‚ ¾æÒ . «¾Ò’öBº * Ú'b‚ &–’ &Ë 12"ræ n;‚ 8j ¾æÚî . −20oC &˖öBê ËO»ö ç& ìš &ËV*š ^Úî >ƒ Î ¾Ò’öB a8š Ã&~&b– &–’öBº ÷, bag˾ҒöBº 15" ã"¦V a8š F~'b‚ Nš&. Ë89ª ; &Vò 10 gj Ã~> 100 mLf bÎ Ê Schultz (6)~ O»ö 8¢ Likens & Nickerson Ë~¢ ÒÏ~  ³>ÃVÃ~»b‚ ËVWªj 2* ÿn ÷~& . ³ >ÃVÃ~»ö ~šB áÚê ò~ ËVWª~ ·'ž æ. Table 1. Changes in “a” values of minced ginger packed in three types of containers during storage at 5oC Color value Storage time (weeks) Bottle 0 4 8 12 16 1). Additives1). Control 2). d. 2.47 3.24c 3.61b 3.88a 3.90b. Bag. Tube. c. c. 2.71 3.64b 3.84b 3.62a 3.89a. 2.69 2.96b 3.41a 3.46a 3.58a. Bottle a. 2.86 2.84a 2.95a 2.99a 3.08a. Bag. Tube. b. 2.04a 2.24a 2.77a 2.63a 2.98a. 2.67 2.62b 2.81ab 2.94ab 3.07a. Additives: control+L-cysteine 0.2%+sodium benzoate 0.1%+NaCl 2%+xanthan gum 0.1%. Means with the same letter in the same column are significantly different as determined by Duncan’s multiple range test (p<0.05).. 2)a,b,c,d.

(7) ïÿ;b‚ B–‚ &V~ &Ë 7 ®îæz. 945. Table 2. Changes in “a” values of minced ginger packed in three types of containers during storage at −20oC Color value Storage time (weeks) Bottle 0 15 30 45 60. Additives1). Control 2). c. 2.13 2.86b 3.07ab 3.13a 3.27a. Bag d. 2.29 3.31c 3.54b 3.59b 3.83a. Tube. Bottle. b. b. 2.17 2.26b 2.25b 2.30b 2.81a. 2.07 2.16b 2.13b 2.28ab 2.48a. Bag b. 2.15 2.34ab 2.37ab 2.31ab 2.49a. Tube 2.06b 2.13b 2.08b 2.20ab 2.32a. 1). Additives: control+L-cysteine 0.2%+sodium benzoate 0.1%+NaCl 2%+xanthan gum 0.1%. Means with the same letter in the same column are significantly different as determined by Duncan’s multiple range test (p<0.05).. 2)a,b,c,d. Table 3. Changes in total free sugar contents of minced ginger packed in three types of containers during storage at 5oC Free sugar(%) Storage time (weeks) Bottle 0 4 8 12 16. Additives1). Control 2). a. 0.68 0.48b 0.36bc 0.37bc 0.34c. Bag a. 0.66 0.43b 0.37bc 0.39bc 0.34c. Tube. Bottle. a. a. 0.69 0.49b 0.43b 0.37b 0.37b. 0.66 0.52b 0.50b 0.48b 0.49b. Bag. Tube. a. 0.68a 0.58ab 0.54b 0.55b 0.54b. 0.67 0.46b 0.43b 0.42b 0.41b. 1). Additives: control+L-cysteine 0.2%+sodium benzoate 0.1%+NaCl 2%+xanthan gum 0.1%. Means with the same letter in the same column are significantly different as determined by Duncan’s multiple range test (p<0.05).. 2)a,b,c. Table 4. Changes in total free sugar contents of minced ginger packed in three types of containers during storage at -20oC Free sugar(%) Storage time (weeks) Bottle 0 15 30 45 60. Additives1). Control 2). a. 0.64 0.59ab 0.56b 0.41c 0.37c. Bag a. 0.63 0.58b 0.54b 0.37c 0.35c. Tube a. 0.64 0.61a 0.57a 0.42b 0.38b. Bottle a. 0.65 0.62a 0.62a 0.46b 0.47b. Bag. Tube. a. 0.65a 0.65a 0.61ab 0.50ab 0.49b. 0.65 0.61ab 0.60ab 0.49bc 0.47c. 1). Additives: control+L-cysteine 0.2%+sodium benzoate 0.1%+NaCl 2%+xanthan gum 0.1%. Means with the same letter in the same column are significantly different as determined by Duncan’s multiple range test (p<0.05).. 2)a,b,c. ¾V ·~&b¾ Â2˾ҒöBº ž ˾Ғ. a8~ Ã&š jò~& . «¾Ò’öBº &–’ & Ë 45"ræ a8~ æz& n;'b‚ ¾æÚî . f (11)f ;j 6" ÿn &Ë~&j H 'ïêž a8" ïêž b8š Ã&~&b–, ‚šïêº &ËV*š ^Úî >ƒ .æê Ã&ö ~‚ ïê~ Ã&*çš ¾æÂ   ~  ’Ö"f ?f ãËj ¾æÚî . $‚ f (12) f f~¢ PE jª" rª '[jªb‚ Ë~ ®îG ;j ‚ Ö" '[jª~ ãÖ PEjª~ ãÖ Öê~ à && Ô² ¾æÒb–, ïê~ æzº '[jª ˾Ғ& PEjª¾Ò’ n;'ž 8j ¾æÞ  ~& . šº &Ë 5 FÛ 7 >ª¶ 5 Ö²R"¢ Oæ† > ®º ' [jª ËO»š Fê Fæö 'Ëj  bÒ¢ ÒòB . FÒ Žï~ æz ïÿ&Ë(−20oC)‚ öò ;b‚ &V B– ê 5oC &Ë ö 16" ÿn &Ë FÒ Žï æz¢ G;‚ Ö"º. Table 3ö ¾æÞ :f ?~ . FÒ Žïf &Ë .Vf j v~&j H &ËV*š ^Úî>ƒ ËO»ö ç&ìš 6 ²~&b– &Ë 16"& ã"~š &–’öB ÷Ëf 50.0%, bagËf 48.4%, Â2Ëf 46.3%O 6²~& . &Ë.Vf jv~ " H &Ë 4" ã"¦V FÒ Žï~ F~'ž N šº ¾æ¾V · ®b¾ Â2˾ҒöBº FÒ Ž ï~ 6²& ž ˾Ғ &Ë 16"ræ F~'ž æ z& ¾æ¾æ p~ . «¾Ò’öBº ËO»ö &êìš &–’f jv~š &Ë 16"& ã"~ê jò‚ FÒ Ž ï~ 6²¢ ¾æÚîb–, ß® Â2˾Ғº &Ë 4"r æ FÒ Žï~ F~'ž Nš& ¾æ¾æ pj ž Ë Ò n;‚ FÒ Žï~ æz¢ ¾æÚî . −20oC &Ë  &Ë 60"& ã"~š &–’öB ÷Ëf 46.8%, bag Ëf 44.4%, Â2Ëf 40.6%‚ FÒ Žïf 6²~&. (Table 4). ¾ Â2˾ҒöBº FÒ Žï~ 6² š ž ˾Ғ 30" &Ë¢ræ 6² š jò~&. . «¾Ò’öBº ËO»ö &êìš &–’f jv~š.

(8) ‚“®"²æ B 36 ² B 6 ^ (2004). 946. Table 5. Changes in total free amino acid contents of minced ginger packed in three types of containers during storage at 5oC Total free amino acid(mg%) Storage time (weeks) Bottle 0 4 8 12 16. Additives1). Control 2). Bag a. 434.0 418.6ab 415.8ab 405.9bc 386.4c. Tube a. 437.5 419.6ab 403.8bc 397.5cd 379.8d. a. Bottle. 436.5 424.6ab 417.7abc 403.7bc 391.1c. a. 435.1 426.4ab 420.9abc 415.5bc 410.1c. Bag. Tube a. 437.3 423.3ab 421.4ab 414.0b 411.3b. 436.4a 427.2ab 423.7ab 419.4ab 414.2b. 1). Additives: control+L-cysteine 0.2%+sodium benzoate 0.1%+NaCl 2%+xanthan gum 0.1%. Means with the same letter in the same column are significantly different as determined by Duncan’s multiple range test (p<0.05).. 2)a,b,c,d. Table 6. Changes in total free amino acid contents of minced ginger packed in three types of containers during storage at -20oC Total free amino acid (mg%) Storage time (weeks) 0 15 30 45 60. Additives1). Control Bottle 432.4a 415.1ab 410.1b 401.3b 374.7c. 2). Bag 430.3a 410.4b 405.7b 396.5c 371.2d. Tube 433.0a 417.3a 411.5b 405.3b 380.4c. Bottle 433.2a 428.4ab 421.0bc 416.1cd 410.2d. Bag 436.3a 422.4b 420.2b 410.2c 404.3c. Tube 436.3a 432.5ab 426.4abc 417.9bc 413.9c. 1). Additives: control+L-cysteine 0.2%+sodium benzoate 0.1%+NaCl 2%+xanthan gum 0.1%. Means with the same letter in the same column are significantly different as determined by Duncan’s multiple range test (p<0.05).. 2)a,b,c,d. &Ë 60"& ã"~š ÷Ëf 27.7%, bagËf 27.8%,  2Ëf 24.6%O 6²~&b¾ FÒ Žï~ 6²š &Ë 30"ræ F~'ž Nš& ¾æ¾æ pj «¾Ò’öBº & –’ n;‚ FÒ Žïj ¾æÚî . FÒjžÖ Žï~ æz ïÿ&Ë(−20oC)‚ öò ;b‚ &V B– ê 5oC &Ë ö 16" ÿn &Ë  FÒ jžÖ Žï æz¢ G;‚ Ö "º Table 5ö ¾æÞ :f ?~ . ËO»ê‚~ C FÒ jžÖ Žïf &Ë .Vf jv~&j H &ËV*š ^Ú î>ƒ 6²~º ãËj ¾æÚîb–, &Ë 16"& ã"~š &–’öB ÷Ëf 10.9%, bagËf 13.1%, Â2Ëf 10.4%O 6² ~& . Â2˾Ғf ÷˾Ғº &– ’öB &Ë 8"ræ bag˾Ғ FÒjžÖ~ Žï š ¢;‚ >&j Fæ~& . «¾Ò’öº bag, ÷˾ Ғ& &Ë 8"ræ FÒjžÖ Žï~ 6²š jò® ¾ æÒb¾ Â2˾Ғº &Ë 12" ræ n;'ž ãËj ¾æÚî . −20oC &Ë (Table 6) &Ë 60"& ã"~š & –’öB ÷Ëf 13.3%, bagËf 13.7%, Â2Ëf 12.1% O 6²~& . Bag˾ҒöBº &Ë 15" ã"¦V F ÒjžÖ Žï~ 6² š F~'b‚ ¾æÒb– *Ú' b‚ &Ë 60"¢ræ ž ËO» jžÖ Žï~ 6 ² š ¸~ . «¾Ò’öº &Ë 60"& ã"~š ÷Ë f 5.3%, bagËf 7.3%, Â2Ëf 5.1%~ 6²ïj ¾æ ÚÚ bag˾ҒöB FÒjžÖ Žï~ 6²Nš &Ë ¸~ . ¾ Â2˾Ғº &Ë 30"ræ FÒjžÖ Žï~ F~'ž Nš& ¾æ¾æ pj ž ËÒ n; ‚ ãËj ¾æÚî . jžÖ~ &Ë 7 6²º .ïz >wö V‚  rJ ^ ®b¾ Montgomery (13)º cysteine" ?f ŽjžÖj. Î&~š .ïz >w" Ë&~¢ ÛB‚  ~  ;ö .æÛB Î&bj Î&‚ þ–öBº jžÖ Žï ~ 6²Nš &–’ ÛBNj  "î . šç" ?š ; &V B®öB amino-carbonyl >wb‚ ž‚ ®îæzºžj «~ " H(Fig. 1-4) &–’ « ¾Ò’öB FÒ" FÒjžÖ Žï~ 6²š jò~ &b– 8¢B ïN~ æzê «¾Ò’öB jò~² à &Nj r > ®î . š‚ Ö"º Wiley(14)f VamosVigyazo(15)& "j~º >{ ê &¾Ò>º ";öB –ç š ¶ç>Ú Öz' .æj AV ’Ö– $‚ š‚ ";ö B B~º .æ>wf B®~ ®î¶çö 7º‚ ºžš¢  ~& . 8¢B &V B®ö «'ž Î&bj Î& Žb‚Ž Î&b *~ ç^·Ïb‚ ž‚ .æ>w~ ®îæ z¢ ÛB~º V.¶ò& >Ò¢ ÒòB . Ë89ª Žï~ æz ïÿ&Ë(−20oC)‚ öò ;b‚ &V B– ê 5, −20oC &Ëö 16, 60" ÿn &Ë  "º ËVWª Žï~ æz ¢ G;‚ Ö"º Table 7-10ö ¾æÞ :f ?~ . ËO» ê‚~ "º ËVWª~ Žïf &Ë .Vf jv~&j H & ËV*š ^Úî>ƒ 6²~º ãËj ¾æÚîb¾ &–’ö Bº ÷˾Ғf bag˾Ғ Â2˾ҒöB ËVWª~ Žï~ 6² š Ô² ¾æÒ . ¾ «¾Ò ’öBº &ËV*š ^Ú^ê ËO»ö &êìš &–’. Ôf ËVWª Žï~ ¶ j ¾æÚ "î . &Ú'b‚ ; &V~ ÷Ë, bagË Â2Ë O»š ËVWª ~ 6²š Ô² ¾æÒb–, Î&bj Î&‚ «¾Ò–ö B Â2Ë O»š ËVWª~ 6²j &–’ jò~ ² ¾æÚÚ ÷, bagË O» Â2ËO»š ËVWª ®î~ n;Wj ¾æÚî ..

(9) ïÿ;b‚ B–‚ &V~ &Ë 7 ®îæz. 947. Fig. 1. Changes in ∆E, total free sugar (TFS) and total free amino acid(TFAA) contents of minced ginger packed in three types of containers during storage at 5oC. ∆E: -ù-: bag, -þ -: bottle, - -: tube. TFS: ÃùÃ: bag, ÃþÃ: bottle, à Ã: tube. TFAA: -ø-: bag, -ý-: bottle, -ÿ-: tube.. Fig. 3. Changes in ∆E, total free sugar (TFS) and total free amino acid (TFAA) contents of minced ginger packed in three types of containers during storage at −20oC. ∆E: -ù-: bag, -þ -: bottle, - -: tube. TFS: ÃùÃ: bag, ÃþÃ: bottle, à Ã: tube. TFAA: -ø-: bag, -ý-: bottle, -ÿ-: tube.. Fig. 2. Changes in ∆E, total free sugar (TFS) and total free amino acid (TFAA) contents of minced ginger with additives packed in three types of containers during storage at 5oC. ∆E: -ù-: bag, -þ -: bottle, - -: tube. TFS: ÃùÃ: bag, ÃþÃ: bottle, à Ã: tube. TFAA: -ø-: bag, -ý-: bottle, -ÿ-: tube.. Fig. 4. Changes in ∆E, total free sugar (TFS) and total free amino acid (TFAA) contents of minced ginger with additives packed in three types of containers during storage at −20oC. ∆E: -ù-: bag, -þ -: bottle, - -: tube. TFS: ÃùÃ: bag, ÃþÃ: bottle, à Ã: tube. TFAA: -ø-: bag, -ý-: bottle, -ÿ-: tube.. š‚ ãËf ;j 5Bú ÿn &Ë~šB &Ë 7 ËV Wª~ æz¢ –Ò‚ :, &ËV*š ^Úöö 8¢ sesquiterpene hydrocarbons 5 oxygenated sesquiterpene" ?f bî~ –Wj& 6²~º ãËj ž Lee(16)~ f ?f ãËj ¾æÚî . $‚ Sakamura(17)º ¢Ö ;~ &Ë 7 geraniol" geranyl acetate Wªf *&® 6²~&  ‚ : ® Ú,  þ" ?f ãËj ¾æÚî . ¾ ; &Vö Ž Î&bj Î&  .æ>w öò jî¢ ®~~ Ë ê Fæ† > ®  Montgomery(13)º ~  þ~ Î&b Î&–" ?f ãËj ¾æÚî . $‚ ®~ .æ> wb‚ ž‚ Ë ¶ (18)ê Ö²(19) $º >ª‚Wê(20)f &7‚ &Nš ®  >Ú ®bæ‚, >ªR"ê& Ôf rª '[jªb‚ >Ú®º Â2˾Ғ& &Ë Ë ~ æzj '² ¾æÚî .. ã"¦V F~' N𢠾æÚV ·~& . *Ú'ž F^ êº &–’öB &Ë 8" ã"¦V Nš& ¾æÒb¾ Â2 ˾Ғº ž ˾Ғ  F^ê~ ¿ê& ¸² ¾ æÒ . «¾Ò’öBº ïz, šöBº &Ë 12"ræ, F ^êöBº &Ë 8"ræ n;‚ ãËj ¾æîb– ß®  2˾Ғº &Ë 12"ræ F^ê~ F~'ž Nš& ¾ æ¾æ p~ . −20oC &˖öBº ïzö &‚ &Ë' ï& º &–’öB &Ë 45"ræ ËO»ê‚ ç&ìš F~' Nš& ¾æ¾æ p~b¾ šö &šBº &Ë 15"¦V F ~' N𢠾æÚV ·~& . *Ú'ž F^êöBê & –’öB &Ë 15"¦V F~'ž Nš& ¾æÒb¾ *Ú' b‚ «~ š &Ë 60"& ã"~š Â2˾Ғf ÷˾Ғº bag˾Ғ F^ê~ ¿ê& ¸² ¾ æÒ . «¾Ò’öB ïzö &‚ ï&º bag, Â2Ë O » ÷ËO»öB 45"¦V F~' N𢠾æÚV  · ~&b–, šöBº 30", F^êöBº bagËj Bž ~š 45"ræ F~'ž Nšº ¾æ¾æ p~ . &–’. º Î&bj bÎ&‚ «¾Ò’öB ®î~ n;Wš ¸ ² ¾æÒb–, Â2Ë O»š bag, ÷˾Җ n; 'ž ®îj Fæ† > ®j ©b‚ ÒòB . š‚ ãËf "j~öB ®zæOÖf hexenal ~ *. Ëï& ïÿ&Ë(−20oC)‚ öò ;b‚ &V B– ê 5oC &Ë ö 16" ÿn, −20oC &Ëö 60" ÿn &Ë  &Ë' ß Wj G;‚ Ö"º Table 11-12ö ¾æÞ :f ?~ . ïz ö &‚ &Ë' ï&º &–’öB &Ë 12"ræ ËO»ö ç&ìš F~' Nš& ¾æ¾æ p~b¾, šº &Ë 8".

(10) ‚“®"²æ B 36 ² B 6 ^ (2004). 948. Table 7. Changes in main volatile component contents of minced ginger packed in bottles, bags, and tubes during storage at 5oC Peak area (%) Storage time (weeks) Flavor compounds α-Pinene Camphene β-Phellandrene 1,8-Cineole Neral Zingiberene γ-Bisabolene Geranial Citronellol β-Bisabolene. Bottle. Bag. Tube. 0. 16. 0. 16. 0. 16. 0.38 6.91 9.73 0.46 0.14 22.76 4.94 5.23 5.20 8.28. 0.33 6.71 7.04 0.35 0.18 19.85 4.86 4.79 4.05 7.42. 0.32 6.95 9.63 0.40 0.12 23.50 4.86 5.58 5.13 8.30. 0.27 6.79 9.37 0.39 0.19 20.22 4.46 5.32 4.58 6.87. 0.35 7.70 10.15 0.48 0.12 23.10 4.30 5.62 5.06 7.02. 0.30 6.87 7.40 0.35 0.16 23.37 4.04 5.26 4.35 6.95. Table 8. Changes in volatile component contents of minced ginger with additives1) packed in bottles, bags, and tubes during storage at 5oC Peak area (%) Storage time (weeks) Flavor compounds α-Pinene Camphene β-Phellandrene 1,8-Cineole Neral Zingiberene γ-Bisabolene Geranial Citronellol β-Bisabolene. Bottle. Bag. Tube. 0. 16. 0. 16. 0. 16. 0.38 7.21 9.83 0.45 0.10 22.90 5.04 5.74 4.72 8.09. 0.29 7.06 10.11 0.38 0.09 20.87 4.87 5.46 4.40 7.58. 0.42 7.07 9.39 0.57 0.12 22.47 5.25 5.40 4.99 8.04. 0.37 6.79 9.21 0.47 0.08 21.66 4.81 4.92 4.43 7.52. 0.38 6.93 8.76 0.45 0.17 23.56 5.07 4.74 4.88 7.24. 0.32 6.83 8.25 0.39 0.12 23.30 4.75 4.48 4.38 6.42. 1). Additives: control+L-cysteine 0.2%+sodium benzoate 0.1%+NaCl 2%+xanthan gum 0.1%.. Table 9. Changes in main volatile component contents of minced ginger packed in bottles, bags, and tubes during storage at -20oC Peak area (%) Storage time (weeks) Flavor compounds α-Pinene Camphene β-Phellandrene 1,8-Cineole Neral Zingiberene γ-Bisabolene Geranial Citronellol β-Bisabolene. Bottle. Bag. Tube. 0. 60. 0. 60. 0. 60. 0.29 6.66 9.88 0.44 0.16 22.88 5.04 5.67 4.93 8.55. 0.26 6.12 8.49 0.38 0.11 20.41 4.69 4.86 4.13 7.29. 0.30 7.00 9.65 0.45 0.15 22.75 5.00 5.81 4.83 8.40. 0.24 6.23 8.65 0.37 0.10 20.11 4.57 4.70 4.09 7.69. 0.31 6.81 10.42 0.41 0.17 23.17 5.03 5.40 4.98 7.74. 0.26 6.01 9.41 0.39 0.13 22.59 4.70 4.86 4.53 6.74. ’bî‚ ·Ï~ W? 7š¾ >{ ê &Ë  β ö ~ ~ š¢ W~–,  7 ®zæO֞ linoleic acidf linolenic acid& lipoxygenaseö ~šB >Özb‚ B ê ž β~ *šö ~šB šbîš W>º ©b‚ (21) B : ®Ú &Ëï& 7 šf F^êö 'Ëj  bÒ¢. ÒòB . šº "j~öB~ æîŽïf &ڂ 'b¾ ãê, ËV, ï,   " ~ ®î 5 &ËÒö ’² 'Ëj ~ º ºžb‚ (22)B ÚÏ" ¢¦ ¢~~º ãËj ¾æÚ î . $‚ Park (23)f 6j &&ê Òö†2 jª" ¾ ¢†'[ jª, rª'[ jªb‚ Ë~ 20oCö 5Bú.

(11) ïÿ;b‚ B–‚ &V~ &Ë 7 ®îæz. 949. Table 10. Changes in main volatile component contents of minced ginger with additives1) packed in bottles, bags, and tubes during storage at -20oC Peak area (%) Storage time (weeks) Flavor compounds α-Pinene Camphene β-Phellandrene 1,8-Cineole Neral Zingiberene γ-Bisabolene Geranial Citronellol β-Bisabolene. Bottle 0 0.36 7.35 9.92 0.60 0.14 23.86 4.39 4.94 4.87 8.31. Bag 60 0.35 7.06 9.81 0.50 0.14 23.31 4.14 4.72 4.52 8.03. 0 0.33 6.64 9.81 0.54 0.17 22.88 5.14 5.55 4.98 8.14. Tube 60 0.29 6.64 9.97 0.42 0.14 20.52 4.90 5.47 4.36 7.73. 0 0.39 7.02 10.10 0.55 0.17 24.06 4.76 5.23 4.75 7.47. 60 0.36 6.79 9.59 0.43 0.14 21.83 4.31 4.76 4.33 6.85. 1). Additives: control+L-cysteine 0.2%+sodium benzoate 0.1%+NaCl 2%+xanthan gum 0.1%.. Table 11. Sensory color, off-odor and overall preference of minced ginger packed in three types of containers during storage at 5oC A. Color Color Storage time (weeks). Bottle. Bag Additives1). Control. Control. Tube Additives. Control. a. a. a. a. a. 6.25B. Additives. 0 4 8 12 16. 2). 6.52 6.40a 6.30a 6.15ab 5.90b. 6.70 6.45a 6.40a 6.43a 6.10b. 6.58 6.45a 6.22ab 6.10ab 5.86b. 6.75 6.55a 6.50a 6.43a 6.15b. 6.55 6.50a 6.48a 6.20a 5.97b. 6.80a 6.78a 6.60a 6.45a 6.50a. Mean. 3). 6.41B. 6.24B. 6.47B. 6.34A. 6.62A. B. Off-odor Off-odor Storage time (weeks). Bottle Control. Bag Additives. Control. Tube Additives. Control. Additives. 0 4 8 12 16. a. 5.40 4.95ab 4.52b 4.37b 3.70c. a. 5.50 5.40a 5.35a 5.38a 4.72b. a. 5.45 4.97ab 4.45b 3.82c 3.35c. a. 5.65 5.45a 5.50a 5.35a 4.50b. a. 5.52 4.95ab 4.75b 4.55b 4.15c. 5.60a 5.58a 5.50a 5.40a 4.85b. Mean. 4.58B. 5.27B. 4.40B. 5.29B. 4.78A. 5.38A. C. Overall preference Overall preference Storage time (weeks). Bottle Control. 0 4 8 12 16 Mean 1). a. 5.94 5.33a 4.90b 4.62b 3.80c 4.91B. Bag Additives a. 5.75 5.40ab 5.35ab 5.10b 5.02b 5.32A. Control a. 5.94 5.58a 4.75b 4.03c 3.85c 4.38B. Tube Additives a. 5.70 5.55ab 5.50ab 5.25b 5.15b 5.43A. Control a. 6.03 5.65a 4.92b 4.70b 4.25c 5.11A. Additives 5.64a 5.35ab 5.45ab 5.30ab 5.10b 5.37A. Additives: control+L-cysteine 0.2%+sodium benzoate 0.1%+NaCl 2%+xanthan gum 0.1%. Means with the same letter in the same column are significantly different as determined by Duncan’s multiple range test (p<0.05). 3)A,B Means with the same letter in the same row are significantly different as determined by Duncan’s multiple range test (p<0.05). 2)a,b,c.

(12) ‚“®"²æ B 36 ² B 6 ^ (2004). 950. Table 12. Sensory color, off-odor and overall preference of minced ginger packed in three types of containers during storage at -20oC A. Color Color Storage time (weeks). Bottle. Bag 1). Tube. Additives. Control. Additives. Control. Additives. 0 15 30 45 60. 2). 6.80a 6.50a 6.35ab 6.30b 6.15b. 6.50a 6.24a 6.20a 6.10a 4.10b. 6.85a 6.60a 6.40ab 6.45ab 6.20b. 6.56a 6.34a 6.25a 6.15a 5.13b. 6.75a 6.65a 6.50ab 6.30ab 6.10b. Mean. 3). 6.42A. 5.82B. 6.50A. 6.08A. 6.46A. Control 6.52a 6.35a 6.20a 6.12a 5.10b 6.05A. B. Off-odor Off-odor Storage time (weeks). Bottle Control. Bag Additives. Tube. Control. Additives. Control. Additives. 0 15 30 45 60. a. 5.55 4.85b 4.46b 4.03c 4.00c. a. 5.60 5.60a 5.20ab 5.15b 4.80b. a. 5.45 4.83b 4.40b 3.90c 3.65c. a. 5.70 5.35ab 5.60a 5.25b 4.85c. a. 5.50 4.80b 4.62b 4.20c 4.15c. 5.65a 5.55a 5.30a 5.25a 4.75b. Mean. 4.58A. 5.27A. 4.45A. 5.35A. 4.65A. 5.30A. C. Overall preference Overall preference Storage time (weeks). Bottle. Bag. Tube. Control. Additives. Control. Additives. Control. Additives. 0 15 30 45 60. 5.86a 5.40b 5.13b 5.00b 4.63c. 5.50a 5.35ab 5.25ab 5.20ab 5.05b. 5.84a 5.41b 5.10b 4.90b 4.52c. 5.78a 5.48ab 5.40ab 5.25b 5.00b. 5.90a 5.45b 5.15b 5.05b 4.65c. 5.60a 5.30ab 5.42a 5.35ab 5.00b. Mean. 5.20A. 5.27A. 5.15A. 5.38A. 5.24A. 5.33A. 1). Additives: control+L-cysteine 0.2%+sodium benzoate 0.1%+NaCl 2%+xanthan gum 0.1%. Means with the same letter in the same column are significantly different as determined by Duncan’s multiple range test (p<0.05). 3)A,B Means with the same letter in the same row are significantly different as determined by Duncan’s multiple range test (p<0.05). 2)a,b,c. &Ë ê ž&, –ç6, , Gš B j ï&‚ Ö" r ª ^¢ '[ þ’º &Ë 5Bú êöê &Ëï& , æ ËÒ þ’ö jšB Ö>®  ~  þÖ"f ?f ãËj ¾æÚî .. &8 *®~ ®î>« ËO»ê ; &V~ ®îf 5oC &˖ &–’öB & Ë' ïzf 12", šº 4", F^êº 4"ræ F~'ž N š& ¾æ¾æ p~ . $‚ «¾Ò’öB ïz" šº 12 ", F^êº 8" &Ë¢>ræ F~'ž Nšº ¾æ¾æ p ~ . ËO»ê‚º &–’öB ïz" š, F^êöB ' ' Â2˚ bag, ÷Ë" F~'ž N𢠾æÚî . « ¾Ò’º ïz" šöB &–’f ?f ãËb‚ Â2 ˚ F~' N𢠾æÚîb¾, F^êº ËO»ö &ê ìš F~'ž Nš& ¾æ¾æ p~ . −20oC &˖ &–’ öBº &Ë' ïzf 45"ræ ®îš &Ë'b‚ n;~& b¾ š, F^êº &Ë 15"¦V F~' N𢠾æÚÚ. ; &V¢ −20oC &Ëö &Ë~š ®î>«š 15" š~ ‚ .çB  ÒòB . ¾ «¾Ò’öBº ïz, š, F^ê Îv &Ë 30"ræ F~'ž Nš¢ ¾æ¾æ p~b – Â2ËO»f &Ë 45"ræê F~'ž N𢠾æÚ æ pj jª, ÷Ë ; &V~ ®îš  n;'ž ©b‚ ÒòB . šf ?f Ö"¢ Æ&‚ ïÿ;j šÿ~ &V¢ B –‚ ê 5oC &ËNêöBº &–’~ ãÖ 4" &ï ®îš Fæ>¾ «¾Ò’öBº 12" ;êræ ®î>«š Fæ> º ©b‚ ï&>î . −20oC &˖öBº &–’öB šf F^ê~ &~‚ 15" š~~ ®î>«š G;>îb¾, « ¾Ò’öB~ ; &Vº &Ë 30"ræ ®î>«š n;' b‚ Fæ>î, ß® Â2Ë O»f &Ë 45"ræê ®î >«š Fæ>î .. º. £.  ’öBº ïÿ &ËB ;b‚ &V¢ ËO»ê.

(13) ïÿ;b‚ B–‚ &V~ &Ë 7 ®îæz. ‚ B–~ Fۖšö 8ž ®îæz¢ –Ò‚ Ö"¢ º£ ~š r" ? . 5, −20oC &ËNêöB &V~ ‚šïê ~ æzº «¾Ò’& &–’ n;'šîb–, C FÒ  Žï" jžÖ Žïf &ËV*š ^Úî>ƒ 6²~& b¾, Â2˾ҒöBº ž ˾Ғ 6² š jò~& . ËVWª Žï~ æzº Â2Ë O»" Î&b j «'b‚ Î&‚ ¾Ò–öB ËVWª Žï~ 6² š jò~² ¾æÒ . &Ë' ï&º 5oC &˖öB «¾Ò’ & &–’ ïz, šöB &Ë 12"ræ, F^êöB & Ë 8"ræ n;‚ ãËj ¾æîb–, ß® Â2˾Ғº &Ë 12"ræ F^ê~ F~'ž Nš& ¾æ¾æ p~ . −20oC &˖öBº &–’öB &Ë 45"ræ ËO»ê‚ ç&ìš ïzöB F~' Nš& ¾æ¾æ p~b¾ šö &šBº &Ë 15"¦V F~' Nš& ¾æ¾V ·~& . *Ú'ž F^êº 15" &˦V F~'ž Nš& ¾æÒb – Â2˾Ғf ÷˾Ғº bag˾Ғ  ¸ ² ï&>î . «¾Ò’öBº ïz, š, F^êöB Â2 Ëf &Ë 45"ræ F~N& ¾æ¾æ pj ž Ë¾Ò ’ n;W𠏲 ¾æÒ . ; &V B®~ ®î>« f &–’~ ãÖ 5oCöBº ®îFæV*š 4" ;êö ®" ~æò Î&bj Î&‚ ãÖº 12"ræê ®îj Fæ† > ®º ©b‚ ¾æÒ . −20oC &˖öBº &–’öB šf F^ê~ &~‚ ®î>«š 15";ê‚ ² ¾æÒb¾ Î &bj Î&‚ &Vº &Ë 30"ræ ®îš n;'b‚ F æ>î, ß® Â2Ë O»f &Ë 45"ræê ®îæz& –~ ìÚ n;B ®î>«j ¾æÚî . šç~ Ö"¢ « š " H &ËW𠣂 ;~ ®îæz¢ ‚²z ~V*š ïÿ;b‚ Î&bj Î&‚ ; &V B®j Â2Ë~  B–~š ; &V~ ®îæz¢ ‚²z† > ®j ©b ‚ Òò>î .. 6Ò~  ’º ³â¦ ³âVFBBÒë~ æöö ~š >¯> îb–, šö 6Òãî .. ^. ò. 1. Lee YN. Flora of Korea. Kyohaksa, Seoul, Korea. pp. 1107-1109 (1996) 2. MAF. Agriculture and Forestry Statistical Yearbook 2001. Ministry of Agriculture and Forestry, Seoul, Korea (2001) 3. Enmaya H. Dictionary of Food Science. Tokyo, Japan. pp. 300301(1981). 951. 4. Gancedo MC, Luh BS. HPLC analysis of organic acid and sugars in tomato juice. J. Food Sci. 50: 571-573 (1986) 5. Choi HS. Studies on cooked rice flavor. PhD thesis, Dongguk University, Seoul, Korea (1976) 6. Schultz TH, Flath RA, Mou TR, Eggling SB, Teranishi R. Isolation of volatile components from a model system, J. Agric. Food Chem. 25: 446-449 (1977) 7. Smith RM, Rdoinson JM. The essential oil of ginger from Fiji. Phytochemistry 20: 203-207 (1981) 8. Chen CC, Ho CT. Gas chromatographic analysis of volatile components of ginger oil (Zingiber officinale Roscoe) extracted with liquid carbon dioxide. J. Agric. Food Chem. 36: 322-325 (1988) 9. EPA/NIH. Mass spectral data base USA. The Department of Commerce, Washington DC, USA (1978) 10. SAS Institute, Inc. SAS User’s Guide. Statistical Analysis Systems Institute, Cary, NC, USA (1988) 11. Kim JT, Kim JH, Cung GW. Study on distribution improvement for ginger by drying technology. Ministry of Agriculture and Forestry, Seoul, Korea, GA0259-0113 (2001) 12. Kim YJ, Hong SI, Park NH, Chung TY. Effect of packaging material on quality of Kimchi during storage. Korean J. Food Sci. Technol. 26: 62-67 (1994) 13. Montgomery MW. Cysteine as an inhibitor of browning in pear juice concentrate. J. Food Sci. 48: 951-952 (1983) 14. Wiley RC. Minimally processed refrigerated fruits and vegetables, Chapman & Hall. Inc., New York, USA. pp. 1-14 (1994) 15. Vamos-vigyazo L. Polyphenol oxidase and peroxidase in fruits and vegetables, CRC Crit. Rev. Food Sci. Nutr. 15: 49-127 (1981) 16. Lee BE. Changes in volatile constituents of Zingiber officinale Roscoe Rhizomes during storage. MS thesis, Chonbuk National University, Chonju, Korea (1993) 17. Sakamura F. Changes in volatile constituents of Zingiber officinale rhizomes during storage and cultivation. Phytochemisty 26: 2207-2212 (1987) 18. You BJ, Lee KH, Kim CY, Lee JH. Antioxidant activity of amino acid-xylose browning reaction products. Korean Fish. Soc. Bull. 19: 1-9 (1986) 19. Han YS, Park JY. The microbiological and sensorial properties of frozen bibimbap namul during storage. Korean J. Soc. Food Cook. Sci. 17: 149-155 (2001) 20. Tsai CH, Kong MS, Pan BS. Water activity and temperature effects on nonenzymic browning of amino acids in dried squid and simulated model system. J. Food Sci. 56: 665-670 (1991) 21. Rhee CO, Toshio D, Yukio K. Distribution of lipoxygenase activity among flavors from different milling of wheat. Food Biotech. 2: 442-445 (1993) 22. Matoba J, Hidaka H, Narita H, Kitamura K, Kaizuma N, Kito M. Lipoxygenase-2 isoenzyme is responsible for generation of hexanal in soybean homogenate. J. Agric. Food Chem. 33: 812-815 (1985) 23. Park HW, Koh HY, Park MH. Effect of packaging materials and methods on the storage quality of dried persimmon. Korean J. Food Sci. Technol. 21: 321-325 (1989) (2004j 11ú 5¢ %>; 2004j 12ú 21¢ j).

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