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Mutational spectrum of NF1 gene in Korean patients with neurofibromatosis type 1

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FIG  1.  Genetic  map  of  NF1  gene.  The  NF1  gene  is  located  on  the  chromosome  17q11.2  and  consist  of  60  exons
Table  2.  Primer  sequences,  PCR  product  size  and  their  fragment  analysis  conditions  (  DHPLC  oven  temperature;  This  resolution  temperature  can  be  predicted  by  use  of  DHPLCMelt  software    http://insertion.stanford.edu/melt.html)
FIG  2.  Different  DHPLC  chromatograms  for  NF1  mutations  identified.    Arrow  indicates  chromatograms  originating  from  a  heteroduplex.
FIG  3.  Results  of  direct  sequencing
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