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Multiplex secretome engineering enhances recombinant protein production and purity

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Academic year: 2021

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Table 1 Selection of targets and MiSeq veri fication of knockouts.
Fig. 2 Veri fication of knockouts by mass spectrometry. Supernatant samples prepared from WT (red bars) were subjected to MS and compared to the a 6xKO (green bars), b 11xKO (blue bars) and c 14xKO (gray bars) cell lines with respect to the indicated target
Fig. 3 Knockout cell lines display improved growth and HCP pro file. a Viability and VCD, b HCP profile, c normalized HCP content on day 7, and d normalized speci fic HCP productivity of WT (red), 6xKO (green), 11xKO (blue), and 14xKO (gray) cell lines
Fig. 4 Knockout cell lines display increased productivity and less HCCF protein content
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