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A study on the effect of freezing rate on catalytic activity and molecular structure of L-lactate dehydrogenase

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Academic year: 2021

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Fig. 1. Schematic diagram of the experimental freeze-thawing process. LMF, liquid-mediated freezing
Fig. 2. Schematic diagram of the ice seeding.  The nucleation temperature was -1 o C.
Fig. 3. Schematic diagram  of the liquid-mediated freezing system  (a). T I   indicates  the temperature of isopropyl    alcohol (IPA), and T S  represents the temperature of the enzyme solution
Fig. 4. Freezing curves of L-lactate dehydrogenase (LDH) solution  at different freezing conditions (T I  = x°C, x = -10, -20, -30, -40, and  -50)
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