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Heat shock protein 90 inhibitor decreases collagen synthesis of keloid fibroblasts and attenuates the extracellular matrix on the keloid spheroid model

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Figure 1. Histologic analysis of keloid tissue. (A) H&E staing. Under the light microscope (×12), we found that  keloid tissue had a dense and excessive deposition of collagen
Figure 2. Quantitative analysis of mRNA expression of type I and III collagen. (A) qRT–PCR analysis indicated  that typeI collagen mRNA levels in KFs treated withvarious amounts of 17AAG treatment (2, 5, 10 μM,  2days)was significantly decreased (**p<0.
Figure 3. Immunoblotanalysis of Smad 2/3 complex expression in KFs. (A) 17AAG (5 μM)decreasedthe  expression of Smad 2/3 complex protein in KFs compared with non-treated KFs
Figure 4. Masson’s trichrome staining of the keloid spheroid. (A) Comparison between keloid tissue and  adjacent normal tissue
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